Human/Mouse MYCL1/L‑Myc Antibody

R&D Systems | Catalog # AF4050

R&D Systems
Discontinued Product
AF4050 has been discontinued. View all MYCL1/L-Myc products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MYCL1/L-Myc Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MYCL1/L‑Myc
Gly16-Asn139
Accession # P12524

Specificity

Detects human and mouse MYCL1/L‑Myc in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse MYCL1/L‑Myc Antibody

Detection of Human/Mouse MYCL1/ L-Myc antibody by Western Blot.

Detection of Human/Mouse MYCL1/ L‑Myc by Western Blot.

Western blot shows nuclear extracts of HeLa human cervical epithelial carcinoma cell line, A549 human lung carcinoma cell line, JEG-3 human epithelial choriocarcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse MYCL1/L-Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4050) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for MYCL1/L-Myc at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
MYCL1/L-Myc antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

MYCL1/L‑Myc in HeLa Human Cell Line.

MYCL1/L-Myc was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse MYCL1/L-Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4050) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, blue panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
MYCL1/L-Myc antibody in NIH3T3 Mouse Cell Line by Immunocytochemistry (ICC).

MYCL1/L‑Myc in NIH3T3 Mouse Cell Line.

MYCL1/L-Myc was detected in immersion fixed NIH3T3 mouse embryonic fibroblast cell line using Goat Anti-Human/Mouse MYCL1/L-Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4050) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human MYCL1/L‑Myc by Western Blot

Detection of Human MYCL1/L‑Myc by Western Blot

Differential effects of MYC and NF-kappa B on glycolytic gene and MCT1 levels.A) Promoter region of MCT1 includes MYC and NF-kappa B binding sites. Shading reflects four independent IMR90 DNase I hypersensitivity datasets. B) IMR90 cells stably expressing ST, GFP or p53DD + hTERT (PH) and MCPyV tumor-derived early-region (PHE) with inducible expression of MYC, MYCN or MYCL were treated with dox (+) for 48 hours. Lysates were immunoblotted with the indicated antibodies. C) IMR90 PH and PHE cells inducibly expressing MYC or MYCL were transfected with RelA-specific pooled siRNA (siRelA) or non-targeting siRNA (siCtrl). After 24 hours, cells were refed with dox containing media and lysed after an additional 48 hours. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006020), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human MYCL1/L‑Myc by Western Blot

Detection of Human MYCL1/L‑Myc by Western Blot

Differential effects of MYC and NF-kappa B on glycolytic gene and MCT1 levels.A) Promoter region of MCT1 includes MYC and NF-kappa B binding sites. Shading reflects four independent IMR90 DNase I hypersensitivity datasets. B) IMR90 cells stably expressing ST, GFP or p53DD + hTERT (PH) and MCPyV tumor-derived early-region (PHE) with inducible expression of MYC, MYCN or MYCL were treated with dox (+) for 48 hours. Lysates were immunoblotted with the indicated antibodies. C) IMR90 PH and PHE cells inducibly expressing MYC or MYCL were transfected with RelA-specific pooled siRNA (siRelA) or non-targeting siRNA (siCtrl). After 24 hours, cells were refed with dox containing media and lysed after an additional 48 hours. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006020), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human MYCL1/L‑Myc by Western Blot

Detection of Human MYCL1/L‑Myc by Western Blot

MYC isoforms differentially regulate glycolysis gene expression and ECAR of MCC cells.A) MKL-1 and WaGa cells containing inducible vectors for MYC, MYCN or MYCL were treated with (+) or without (-) dox for 72 hours and lysates were immunoblotted with the indicated antibodies. B) ECAR (mpH/min) of MKL-1 cells inducibly expressing GFP, MYC, MYCN or MYCL after 72 hours of dox addition (minutes). Cells were treated with oligomycin (1 μM) at the indicated time point. *P < 0.05 calculated using unpaired student’s T test between MYC and MYCL samples. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006020), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse MYCL1/L‑Myc Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line, and immersion fixed NIH3T3 mouse embryonic fibroblast cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, A549 human lung carcinoma cell line, JEG-3 human epithelial choriocarcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 3 using AF4050 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MYCL1/L-Myc

MYCL1 is a member of the MYC family that was isolated from small cell lung carcinoma. Like the other members of the MYC family, MYCL1 is a proto-oncogene transcription factor belonging to the helix-loop-helix basic leucine zipper (HLH bzip) family. With its heterodimeric partners, MYCL1 binds to the DNA consensus site 5’ CACGTG 3’. MYCL1 is implicated in controlling a wide range of cellular processes from cellular proliferation to apoptosis.

Long Name

v-Myc Myelocytomatosis Viral Oncogene Homolog 1, Lung Carcinoma Derived

Alternate Names

L-Myc, LMyc

Entrez Gene IDs

4610 (Human); 16918 (Mouse); 298506 (Rat)

Gene Symbol

MYCL

UniProt

P12524

Additional MYCL1/L-Myc Products

Product Documents for Human/Mouse MYCL1/L‑Myc Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse MYCL1/L‑Myc Antibody

For research use only

Citations for Human/Mouse MYCL1/L‑Myc Antibody

Customer Reviews for Human/Mouse MYCL1/L‑Myc Antibody (1)

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  • Human/Mouse MYCL1/L-Myc Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: small cell lung cancer cells
    Species: Human
    Verified Customer | Posted 12/18/2018
    ripa lysis 30ug protein loaded. Not sure which band or is it the right band
    Human/Mouse MYCL1/L‑Myc Antibody AF4050

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Protocols

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