Human/Mouse MYCL1/L-Myc Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse MYCL1/L‑Myc in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human MYCL1/L‑Myc
    Gly16-Asn139
    Accession # P12524
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse MYCL1/
L‑Myc by Western Blot.
Western blot shows nuclear extracts of HeLa human cervical epithelial carcinoma cell line, A549 human lung carcinoma cell line, JEG‑3 human epithelial choriocarcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse MYCL1/L‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4050) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for MYCL1/L‑Myc at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunocytochemistry
MYCL1/L‑Myc in HeLa Human Cell Line. MYCL1/L‑Myc was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse MYCL1/L‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4050) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, blue panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
MYCL1/L‑Myc in NIH3T3 Mouse Cell Line. MYCL1/L‑Myc was detected in immersion fixed NIH3T3 mouse embryonic fibroblast cell line using Goat Anti-Human/Mouse MYCL1/L‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4050) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MYCL1/L-Myc

MYCL1 is a member of the MYC family that was isolated from small cell lung carcinoma. Like the other members of the MYC family, MYCL1 is a proto-oncogene transcription factor belonging to the helix-loop-helix basic leucine zipper (HLH bzip) family. With its heterodimeric partners, MYCL1 binds to the DNA consensus site 5’ CACGTG 3’. MYCL1 is implicated in controlling a wide range of cellular processes from cellular proliferation to apoptosis.

  • Long Name:
    v-Myc Myelocytomatosis Viral Oncogene Homolog 1, Lung Carcinoma Derived
  • Entrez Gene IDs:
    4610 (Human); 16918 (Mouse); 298506 (Rat)
  • Alternate Names:
    BHLHE38; bHLHe38myc-related gene from lung cancer; Class E basic helix-loop-helix protein 38; LMyc; L-Myc; LMYCl-myc-1 proto-oncogene; MYCL; MYCL1; protein L-Myc-1; v-myc avian myelocytomatosis viral oncogene homolog 1, lung carcinoma derived; v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Merkel Cell Polyomavirus Small T Antigen Promotes Pro-Glycolytic Metabolic Perturbations Required for Transformation
    PLoS Pathog., 2016;12(11):e1006020.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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