Human/Mouse NKX6.1 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF5857U Datasheet / COA / SDS

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AF5857U-100UG

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Human/Mouse NKX6.1 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse NKX6.1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human NKX3.1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NKX6.1
Met1-Pro120
Accession # NP_006159

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NKX6.1

NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39‑46 kDa member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a beta -cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces beta -cell mitosis. Human NKX6.1 is 367 amino acids (aa) in length. It contains a repressor region composed of Pro and Ala (aa 136‑173), a DNA binding homeobox (aa 236‑295) and a Glu:Asp‑rich transactivation domain (aa 305‑337). Over aa 1‑120, human NKX6.1 shares 96% aa identity with mouse NKX6.1.

Long Name

NK6 Homeobox 1

Entrez Gene IDs

4825 (Human); 18096 (Mouse); 65193 (Rat)

Alternate Names

Homeobox protein NK-6 homolog A; NK homeo box, family 6, member A; NK homeobox (Drosophila), family 6, A; NK homeobox, family 6, A; NK6 homeobox 1; NK6 transcription factor homolog A; NK6 transcription factor related, locus 1 (Drosophila); NK6 transcription factor related, locus 1; NKX6.1; NKX6-1; NKX6A; NKX6Ahomeobox protein Nkx-6.1