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Human/Mouse NKX6.1 Antibody

Catalog #: AF5857
20 Citations Datasheet / COA / SDS
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AF5857
AF5857-SP

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Detection of Mouse NKX6.1 by Western Blot.
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Citations (20)
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Human/Mouse NKX6.1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse NKX6.1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human NKX3.1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human NKX6.1
Met1-Pro120
Accession # NP_006159
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse NKX6.1 antibody by Western Blot. View Larger

Detection of Mouse NKX6.1 by Western Blot. Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse NKX6.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NKX6.1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry NKX6 by Immunohistochemistry (IHC-P).1 antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

NKX6.1 in Human Pancreas. NKX6.1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human/Mouse NKX6.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5857) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Mouse NKX6.1 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse NKX6.1 by Simple WesternTM. Simple Western lane view shows lysates of beta TC-6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for NKX6.1 at approximately 54 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse NKX6.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5857) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKX6.1

NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39‑46 kDa member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a beta -cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces beta -cell mitosis. Human NKX6.1 is 367 amino acids (aa) in length. It contains a repressor region composed of Pro and Ala (aa 136‑173), a DNA binding homeobox (aa 236‑295) and a Glu:Asp‑rich transactivation domain (aa 305‑337). Over aa 1‑120, human NKX6.1 shares 96% aa identity with mouse NKX6.1.

Long Name
NK6 Homeobox 1
Entrez Gene IDs
4825 (Human); 18096 (Mouse); 65193 (Rat)
Alternate Names
Homeobox protein NK-6 homolog A; NK homeo box, family 6, member A; NK homeobox (Drosophila), family 6, A; NK homeobox, family 6, A; NK6 homeobox 1; NK6 transcription factor homolog A; NK6 transcription factor related, locus 1 (Drosophila); NK6 transcription factor related, locus 1; NKX6.1; NKX6-1; NKX6A; NKX6Ahomeobox protein Nkx-6.1

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Citations for Human/Mouse NKX6.1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. A differentiation protocol for the generation of pancreatic beta-like cells from human embryonic stem cells
    Authors: Xisheng Li, Zhangjing Ma, Kathy O. Lui
    STAR Protocols
  2. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm
    Authors: Mahaddalkar PU, Scheibner K, Pfluger S et al.
    Nature Biotechnology
  3. NKX6.1 Represses Tumorigenesis, Metastasis, and Chemoresistance in Colorectal Cancer
    Authors: Hsin-Hua Chung, Chun-Te Lee, Je-Ming Hu, Yu-Ching Chou, Ya-Wen Lin, Yu-Lueng Shih
    International Journal of Molecular Sciences
  4. Sox5 regulates beta-cell phenotype and is reduced in type 2 diabetes
    Authors: A. S. Axelsson, T. Mahdi, H. A. Nenonen, T. Singh, S. Hänzelmann, A. Wendt et al.
    Nature Communications
  5. Generation of a Novel Nkx6-1 Venus Fusion Reporter Mouse Line
    Authors: Ingo Burtscher, Marta Tarquis-Medina, Ciro Salinno, Silvia Schirge, Julia Beckenbauer, Mostafa Bakhti et al.
    International Journal of Molecular Sciences
  6. Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix
    Authors: Reena Singh, Louise Cottle, Thomas Loudovaris, Di Xiao, Pengyi Yang, Helen E. Thomas et al.
    Stem Cells Translational Medicine
  7. Selective ablation of P53 in pancreatic beta cells fails to ameliorate glucose metabolism in genetic, dietary and pharmacological models of diabetes mellitus
    Authors: C Uhlemeyer, N Müller, M Rieck, J Kuboth, C Schlegel, K Grie beta, TF Dorweiler, S Heiduschka, J Eckel, M Roden, E Lammert, M Stoffel, BF Belgardt
    Molecular Metabolism, 2022-12-05;67(0):101650.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Modeling axonal regeneration by changing cytoskeletal dynamics in stem cell-derived motor nerve organoids
    Authors: WM Seo, J Yoon, JH Lee, Y Lee, H Lee, D Geum, W Sun, MR Song
    Scientific Reports, 2022-02-08;12(1):2082.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  9. A point mutation in the Pdia6 gene results in loss of pancreatic beta-cell identity causing overt diabetes
    Authors: NF Chhabra, AL Amend, A Bastidas-P, S Sabrautzki, M Tarquis-Me, S Sachs, M Rubey, B Lorenz-Dep, A Feuchtinge, M Bakhti, H Lickert, GKH Przemeck, M Hrab? de A
    Molecular Metabolism, 2021-09-04;0(0):101334.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. MIR-NATs repress MAPT translation and aid proteostasis in neurodegeneration
    Authors: R Simone, F Javad, W Emmett, OG Wilkins, FL Almeida, N Barahona-T, J Zareba-Pas, M Ehteramyan, P Zuccotti, A Modelska, K Siva, GS Virdi, JS Mitchell, J Harley, VA Kay, G Hondhamuni, D Trabzuni, M Ryten, S Wray, E Preza, DA Kia, A Pittman, R Ferrari, C Manzoni, A Lees, JA Hardy, MA Denti, A Quattrone, R Patani, P Svenningss, TT Warner, V Plagnol, J Ule, R de Silva
    Nature, 2021-05-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Generation of a Novel Nkx6-1 Venus Fusion Reporter Mouse Line
    Authors: Ingo Burtscher, Marta Tarquis-Medina, Ciro Salinno, Silvia Schirge, Julia Beckenbauer, Mostafa Bakhti et al.
    International Journal of Molecular Sciences
    Species: Mouse
    Sample Types: Cell Lysates, Tissue Homogenates, Whole Tissue
    Applications: Immunohistochemistry, Western Blot
  12. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm
    Authors: Mahaddalkar PU, Scheibner K, Pfluger S et al.
    Nature Biotechnology
  13. Identification of a periodontal pathogen and bihormonal cells in pancreatic islets of humans and a mouse model of periodontitis
    Authors: V Ilievski, PT Toth, K Valyi-Nagy, T Valyi-Nagy, SJ Green, RS Marattil, HW Aljewari, B Wicksteed, NM O'Brien-Si, EC Reynolds, BT Layden, TG Unterman, K Watanabe
    Sci Rep, 2020-06-19;10(1):9976.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture
    Authors: U Diekmann, H Wolling, R Dettmer, I Niwolik, O Naujok, FFR Buettner
    Sci Rep, 2019-01-30;9(1):996.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
    Authors: B Memon, M Karam, S Al-Khawaga, EM Abdelalim
    Stem Cell Res Ther, 2018-01-23;9(1):15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Identification of proliferative and mature ?-cells in the islets of Langerhans
    Nature, 2016-07-11;535(7612):430-4.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P, Western Blot
  17. Hes3 is expressed in the adult pancreatic islet and regulates gene expression, cell growth, and insulin release.
    Authors: Masjkur J, Arps-Forker C, Poser S, Nikolakopoulou P, Toutouna L, Chenna R, Chavakis T, Chatzigeorgiou A, Chen L, Dubrovska A, Choudhary P, Uphues I, Mark M, Bornstein S, Androutsellis-Theotokis A
    J Biol Chem, 2014-11-04;289(51):35503-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. MicroRNA-7a regulates pancreatic beta cell function.
    Authors: Latreille M, Hausser J, Stutzer I, Zhang Q, Hastoy B, Gargani S, Kerr-Conte J, Pattou F, Zavolan M, Esguerra J, Eliasson L, Rulicke T, Rorsman P, Stoffel M
    J Clin Invest, 2014-05-01;124(6):2722-35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Glucose homeostasis is regulated by pancreatic beta -cell cilia via endosomal EphA-processing
    Authors: Francesco Volta, M. Julia Scerbo, Anett Seelig, Robert Wagner, Nils O’Brien, Felicia Gerst et al.
    Nature Communications
  20. Molecular and genetic regulation of pig pancreatic islet cell development
    Authors: Seokho Kim, Robert L. Whitener, Heshan Peiris, Xueying Gu, Charles A. Chang, Jonathan Y. Lam et al.
    Development

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