Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Chicken, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NKX6.1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NKX6.1
Met1-Pro120
Accession # NP_006159

Specificity

Detects human and mouse NKX6.1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human NKX3.1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse NKX6.1 Antibody

Detection of Mouse NKX6.1 antibody by Western Blot.

Detection of Mouse NKX6.1 by Western Blot.

Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse NKX6.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NKX6.1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
NKX6 by Immunohistochemistry (IHC-P).1 antibody in Human Pancreas by Immunohistochemistry (IHC-P).

NKX6.1 in Human Pancreas.

NKX6.1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human/Mouse NKX6.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5857) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Mouse NKX6.1 antibody by Simple WesternTM.

Detection of Mouse NKX6.1 by Simple WesternTM.

Simple Western lane view shows lysates of beta TC-6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for NKX6.1 at approximately 54 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse NKX6.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5857) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse NKX6.1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat pancreas and immersion fixed paraffin-embedded sections of human pancreas

Simple Western

10 µg/mL
Sample: beta TC‑6 mouse beta cell insulinoma cell line

Western Blot

1 µg/mL
Sample: beta TC‑6 mouse beta cell insulinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKX6.1

NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39‑46 kDa member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a beta -cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces beta -cell mitosis. Human NKX6.1 is 367 amino acids (aa) in length. It contains a repressor region composed of Pro and Ala (aa 136‑173), a DNA binding homeobox (aa 236‑295) and a Glu:Asp‑rich transactivation domain (aa 305‑337). Over aa 1‑120, human NKX6.1 shares 96% aa identity with mouse NKX6.1.

Long Name

NK6 Homeobox 1

Alternate Names

NKX6-1, NKX6A

Entrez Gene IDs

4825 (Human); 18096 (Mouse); 65193 (Rat)

Gene Symbol

NKX6-1

UniProt

NP_006159

Additional NKX6.1 Products

Product Documents for Human/Mouse NKX6.1 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse NKX6.1 Antibody

For research use only

Citations for Human/Mouse NKX6.1 Antibody

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Protocols

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