Human/Mouse NKX6.1 Antibody

Catalog #: AF5857
9 Citations Datasheet / COA / SDS

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AF5857
AF5857-SP

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Detection of Mouse NKX6.1 by Western Blot.

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Product Details

Citations (9)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse NKX6.1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse NKX6.1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human NKX3.1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NKX6.1
Met1-Pro120
Accession # NP_006159

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse NKX6.1 by Western Blot. Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse NKX6.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NKX6.1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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NKX6.1 in Human Pancreas. NKX6.1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human/Mouse NKX6.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5857) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Mouse NKX6.1 by Simple Western^TM. Simple Western lane view shows lysates of beta TC-6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for NKX6.1 at approximately 54 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse NKX6.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5857) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKX6.1

NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39‑46 kDa member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a beta -cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces beta -cell mitosis. Human NKX6.1 is 367 amino acids (aa) in length. It contains a repressor region composed of Pro and Ala (aa 136‑173), a DNA binding homeobox (aa 236‑295) and a Glu:Asp‑rich transactivation domain (aa 305‑337). Over aa 1‑120, human NKX6.1 shares 96% aa identity with mouse NKX6.1.

Long Name

NK6 Homeobox 1

Entrez Gene IDs

4825 (Human); 18096 (Mouse); 65193 (Rat)

Alternate Names

Homeobox protein NK-6 homolog A; NK homeo box, family 6, member A; NK homeobox (Drosophila), family 6, A; NK homeobox, family 6, A; NK6 homeobox 1; NK6 transcription factor homolog A; NK6 transcription factor related, locus 1 (Drosophila); NK6 transcription factor related, locus 1; NKX6.1; NKX6-1; NKX6A; NKX6Ahomeobox protein Nkx-6.1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse NKX6.1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Selective ablation of P53 in pancreatic beta cells fails to ameliorate glucose metabolism in genetic, dietary and pharmacological models of diabetes mellitus
Authors: C Uhlemeyer, N Müller, M Rieck, J Kuboth, C Schlegel, K Grie beta, TF Dorweiler, S Heiduschka, J Eckel, M Roden, E Lammert, M Stoffel, BF Belgardt
Molecular Metabolism, 2022;67(0):101650.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Modeling axonal regeneration by changing cytoskeletal dynamics in stem cell-derived motor nerve organoids
Authors: WM Seo, J Yoon, JH Lee, Y Lee, H Lee, D Geum, W Sun, MR Song
Scientific Reports, 2022;12(1):2082.
Species: Human
Sample Types: Organoids
Applications: IHC
MIR-NATs repress MAPT translation and aid proteostasis in neurodegeneration
Authors: R Simone, F Javad, W Emmett, OG Wilkins, FL Almeida, N Barahona-T, J Zareba-Pas, M Ehteramyan, P Zuccotti, A Modelska, K Siva, GS Virdi, JS Mitchell, J Harley, VA Kay, G Hondhamuni, D Trabzuni, M Ryten, S Wray, E Preza, DA Kia, A Pittman, R Ferrari, C Manzoni, A Lees, JA Hardy, MA Denti, A Quattrone, R Patani, P Svenningss, TT Warner, V Plagnol, J Ule, R de Silva
Nature, 2021;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
A point mutation in the Pdia6 gene results in loss of pancreatic beta-cell identity causing overt diabetes
Authors: NF Chhabra, AL Amend, A Bastidas-P, S Sabrautzki, M Tarquis-Me, S Sachs, M Rubey, B Lorenz-Dep, A Feuchtinge, M Bakhti, H Lickert, GKH Przemeck, M Hrab? de A
Molecular Metabolism, 2021;0(0):101334.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Identification of a periodontal pathogen and bihormonal cells in pancreatic islets of humans and a mouse model of periodontitis
Authors: V Ilievski, PT Toth, K Valyi-Nagy, T Valyi-Nagy, SJ Green, RS Marattil, HW Aljewari, B Wicksteed, NM O'Brien-Si, EC Reynolds, BT Layden, TG Unterman, K Watanabe
Sci Rep, 2020;10(1):9976.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture
Authors: U Diekmann, H Wolling, R Dettmer, I Niwolik, O Naujok, FFR Buettner
Sci Rep, 2019;9(1):996.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
Authors: B Memon, M Karam, S Al-Khawaga, EM Abdelalim
Stem Cell Res Ther, 2018;9(1):15.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Identification of proliferative and mature ?-cells in the islets of Langerhans
Nature, 2016;535(7612):430-4.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
MicroRNA-7a regulates pancreatic beta cell function.
Authors: Latreille M, Hausser J, Stutzer I, Zhang Q, Hastoy B, Gargani S, Kerr-Conte J, Pattou F, Zavolan M, Esguerra J, Eliasson L, Rulicke T, Rorsman P, Stoffel M
J Clin Invest, 2014;124(6):2722-35.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC