Human/Mouse Patched 1/PTCH
(First Extracellular Loop) Antibody

Catalog # Availability Size / Price Qty
MAB41051
MAB41051-SP
Detection of Mouse Patched 1/PTCH by Western Blot.
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Product Details
Citations (4)
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Human/Mouse Patched 1/PTCH
(First Extracellular Loop) Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse Patched 1/PTCH (First Extracellular Loop) in direct ELISAs and Western blots. In direct ELISAs and Western blots, 15% cross‑reactivity with recombinant human Patched 2 is observed.
Source
Monoclonal Rat IgG2A Clone # 413220
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Patched 1/PTCH
Glu108-Asp422
Accession # Q61115
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Patched 1/PTCH antibody by Western Blot. View Larger

Detection of Mouse Patched 1/PTCH by Western Blot. Western blot shows lysates of MEF mouse embryonic feeder cells, Neuro-2A mouse neuroblastoma cell line, and mouse brain tissue. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Patched 1/PTCH (First Extracellular Loop) Monoclonal Antibody (Catalog # MAB41051) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Patched 1/PTCH at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Patched antibody in Mouse Spinal Cord by Immunohistochemistry (IHC-Fr). View Larger

Patched in Mouse Spinal Cord. Patched was detected in immersion fixed frozen sections of mouse spinal cord using 10 µg/mL Rat Anti-Human/Mouse Patched 1/PTCH (First Extracellular Loop) Monoclonal Antibody (Catalog # MAB41051) overnight at 4 °C. Tissue was stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Patched 1/PTCH antibody in Human Esophageal Squamous Cell Carcinoma by Immunohistochemistry (IHC-P). View Larger

Patched 1/PTCH in Human Esophageal Squamous Cell Carcinoma. Patched 1/PTCH was detected in immersion fixed paraffin-embedded sections of human esophogeal squamous cell carcinoma tissue using Rat Anti-Human/Mouse Patched 1/PTCH (First Extracellular Loop) Monoclonal Antibody (Catalog # MAB41051) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Patched 1/PTCH

Patched homolog 1 (PTCH, also called PTC, PTC1 or PTCH1) is a 170‑175 kDa member of the Patched family of proteins. It is found on multiple cells types and forms a constitutive, inactivating complex with Smoothened. Upon SHH binding to PTC1, Smoothened is released and initiates downstream signaling. Mouse PTCH is a 12 transmembrane, 1434 aa glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for PTCH has been reported in mice. However, it would appear likely that two or more splice forms exist, with variation occurring over the first 150 aa of the N-terminus. Over aa 108‑422, mouse PTCH shares 97% and 99% aa sequence identity with human and rat PTCH, respectively.

Entrez Gene IDs
5727 (Human); 19206 (Mouse); 89830 (Rat)
Alternate Names
BCNS; BCNSFLJ42602; FLJ26746; HPE7; mes; NBCCS; patched (Drosophila) homolog; Patched 1; patched homolog (Drosophila); patched homolog 1 (Drosophila); patched homolog 1; protein patched homolog 1; PTC; ptc1; PTCH protein +12b; PTCH protein +4'; PTCH protein -10; PTCH; PTCH1; PTCH11

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Citations for Human/Mouse Patched 1/PTCH
(First Extracellular Loop) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Persistent Properties of a Subpopulation of Cancer Cells Overexpressing the Hedgehog Receptor Patched
    Authors: ÁJ Feliz More, A Hasanovic, A Morin, C Prunier, V Magnone, K Lebrigand, A Aouad, S Cogoluegne, J Favier, C Pasquier, I Mus-Veteau
    Pharmaceutics, 2022;14(5):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Inhibition of Gli2 suppresses tumorigenicity in glioblastoma stem cells derived from a de novo murine brain cancer model
    Authors: S Tanigawa, M Fujita, C Moyama, S Ando, H Ii, Y Kojima, T Fujishita, M Aoki, H Takeuchi, T Yamanaka, Y Takahashi, N Hashimoto, S Nakata
    Cancer Gene Therapy, 2021;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development
    Authors: N Sharon, J Vanderhoof, J Straubhaar, J Mueller, R Chawla, Q Zhou, EN Engquist, C Trapnell, DK Gifford, DA Melton
    Cell Rep, 2019;27(8):2281-2291.e5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Defective Wnt-dependent cerebellar midline fusion in a mouse model of Joubert syndrome.
    Authors: Lancaster MA, Gopal DJ, Kim J
    Nat. Med., 2011;17(6):726-31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Secondary Antibodies

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(First Extracellular Loop) Antibody

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(First Extracellular Loop) Antibody
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