Human/Mouse PDGF-AA Quantikine ELISA Kit

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(19 citations)
(1 Review)
  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL), Saliva (25 uL)
  • Sensitivity
    6.29 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva)
  • Specificity
    Natural and recombinant human and mouse PDGF-AA. Recombinant rat PDGF-AA shares 95.8% amino acid sequence identity with recombinant human PDGF-AA.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC22, Quantikine Immunoassay Control Group 7 - Please Inquire
Product Summary
The Quantikine Human/Mouse PDGF-AA Immunoassay is a 4.5 hour solid-phase ELISA designed to measure PDGF-AA in human and mouse cell culture supernates, serum, plasma, and human saliva. It contains E. coli-expressed recombinant human PDGF-AA and has been shown to accurately quantitate the recombinant factor. Results obtained using natural PDGF-AA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human and natural mouse PDGF-AA.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020242424
Mean126469835123417792
Standard Deviation10.938.767.414.850.683
CV%8.78.38.11212.110.5

Recovery

The recovery of PDGF-AA spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 80-107
Platelet-poor Heparin Plasma (n=4) 93 83-108
Platelet-poor Heparin Plasma (n=4) 88 70-100
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of PDGF-AA were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were tested with two reage
Human/Mouse PDGF-AA Quantikine ELISA Kit
Human/Mouse PDGF-AA Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PDGF-AA
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
    • Long Name
      Platelet-derived Growth Factor AA
    • Entrez Gene IDs
      5154 (Human); 25266 (Rat);
    • Alternate Names
      PDGFAA; PDGF-AA;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    19 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
      Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
      J Transl Med, 2017;15(1):90.
      Species: Human
      Sample Type: Cell Lysates
    2. Design, characterization and experimental validation of a compact, flexible pulsed power architecture for ex vivo platelet activation
      Authors: AL Garner, A Caiafa, Y Jiang, S Klopman, C Morton, AS Torres, AM Loveless, VB Neculaes
      PLoS ONE, 2017;12(7):e0181214.
      Species: Human
      Sample Type: Plasma
    3. Combining bevacizumab and chemoradiation in rectal cancer. Translational results of the AXEBeam trial.
      Authors: Verstraete M, Debucquoy A, Dekervel J, Van Pelt J, Verslype C, Devos E, Chiritescu G, Dumon K, D'Hoore A, Gevaert O, Sagaert X, Van Cutsem E, Haustermans K
      Br J Cancer, 2015;112(8):1314-25.
      Species: Human
      Sample Type: Whole Blood
    4. The systemic influence of platelet-derived growth factors on bone marrow mesenchymal stem cells in fracture patients.
      Authors: Tan, Hiang Bo, Giannoudis, Peter V, Boxall, Sally A, McGonagle, Dennis, Jones, Elena
      BMC Med, 2015;13(0):6.
      Species: Human
      Sample Type: Serum
    5. Platelet-derived growth factor alpha mediates the proliferation of peripheral T-cell lymphoma cells via an autocrine regulatory pathway.
      Authors: Piccaluga P, Rossi M, Agostinelli C, Ricci F, Gazzola A, Righi S, Fuligni F, Laginestra M, Mancini M, Sapienza M, De Renzo A, Tazzari P, Gibellini D, Went P, Alviano F, Zinzani P, Bagnara G, Inghirami G, Tripodo C, Pileri S
      Leukemia, 2014;28(8):1687-97.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Platelet rich plasma clot releasate preconditioning induced PI3K/AKT/NFkappaB signaling enhances survival and regenerative function of rat bone marrow mesenchymal stem cells in hostile microenvironments.
      Authors: Peng Y, Huang S, Wu Y, Cheng B, Nie X, Liu H, Ma K, Zhou J, Gao D, Feng C, Yang S, Fu X
      Stem Cells Dev, 2013;22(24):3236-51.
      Species: Human
      Sample Type: Platelet-Rich Plasma
    7. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
      Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
      BMC Cell Biol, 2013;14(0):48.
      Species: Human
      Sample Type: Cell Lysates
    8. Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
      Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
      Diabetologia, 2011;54(9):2358-67.
      Species: Human
      Sample Type: Serum
    9. Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer.
      Authors: Rahbari NN, Schmidt T, Falk CS, Hinz U, Herber M, Bork U, Buchler MW, Weitz J, Koch M
      BMC Cancer, 2011;11(0):286.
      Species: Human
      Sample Type: Serum
    10. Deficiency of liver sinusoidal scavenger receptors stabilin-1 and -2 in mice causes glomerulofibrotic nephropathy via impaired hepatic clearance of noxious blood factors.
      Authors: Schledzewski K, Geraud C, Arnold B, Wang S, Grone HJ, Kempf T, Wollert KC, Straub BK, Schirmacher P, Demory A, Schonhaber H, Gratchev A, Dietz L, Thierse HJ, Kzhyshkowska J, Goerdt S
      J. Clin. Invest., 2011;121(2):703-14.
      Species: Mouse
      Sample Type: Plasma
    11. Mesenchymal stem cells produce Wnt isoforms and TGF-beta1 that mediate proliferation and procollagen expression by lung fibroblasts.
      Authors: Salazar KD, Lankford SM, Brody AR
      Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(5):L1002-11.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    12. Inhaled multiwalled carbon nanotubes potentiate airway fibrosis in murine allergic asthma.
      Authors: Ryman-Rasmussen JP, Tewksbury EW, Moss OR, Cesta MF, Wong BA, Bonner JC
      Am. J. Respir. Cell Mol. Biol., 2009;40(3):349-58.
      Species: Mouse
      Sample Type: BALF
    13. Pharmacodynamics and Pharmacokinetics of the Novel Thrombopoietin Mimetic Peptide RWJ-800088 in Humans.
      Authors: Liem-Moolenaar M, Cerneus D, Molloy C, End D, Brown K, de Kam M, Cohen A, van Hensbergen Y, Burggraaf J
      2008;84(0):481.
      Species: Human
      Sample Type: Plasma
    14. Observations on the microvasculature of bone defects filled with biodegradable nanoparticulate hydroxyapatite.
      Authors: Kilian O, Wenisch S, Karnati S, Baumgart-Vogt E, Hild A, Fuhrmann R, Jonuleit T, Dingeldein E, Schnettler R, Franke RP
      Biomaterials, 2008;29(24):3429-37.
      Species: Porcine
      Sample Type: Plasma
    15. Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer.
      Authors: Mathew P, Thall PF, Wen S, Bucana C, Jones D, Horne E, Oh WK, Morris MJ, Lee YC, Logothetis CJ, Lin SH, Fidler IJ
      Br. J. Cancer, 2008;99(9):1426-32.
      Species: Human
      Sample Type: Plasma
    16. Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor.
      Authors: Dolloff NG, Russell MR, Loizos N, Fatatis A
      Cancer Res., 2007;67(2):555-62.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
      Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N
      Clin. Cancer Res., 2006;12(9):2676-88.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas.
      Authors: Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Nakano T, Okano S, Sata S, Nakagawa K, Yoshino I, Maehara Y, Sueishi K
      Cancer Res., 2005;65(16):7241-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
      Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
      J. Cell. Physiol., 2005;203(1):35-43.
      Species: Human
      Sample Type: Cell Culture Supernates
    Expand to show all 19 Citations
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    Average Rating: 5 (Based on 1 review)

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