Human/Mouse PLA2G4A Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse PLA2G4A in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PLA2G4A
    Met1-Ala749
    Accession # NP_077734
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse PLA2G4A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse PLA2G4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6659) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLA2G4A at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLA2G4A
PLA2G4A (Phospholipase A2 Group IVA; also cytosolic phospholipase A2 alpha/cPLA2 alpha and lysophospholipase) is an 100-110 kDa member of the cytosolic PLA2 family of enzymes. Expression can be induced in select cell types, including endothelium, smooth muscle, macrophages, PMNs, fibroblasts, mast cells and platelets. Tumor cells often serve as sources for cPLA2 alpha activity. cPLA2 alpha is synthesized in the cytosol where, upon activation, it translocates to sites containing cell membranes, including the Golgi, ER and nuclear membrane. This translocation allows for enzymatic action on membrane phospholipids, generating free arachidonic acid that is converted into proinflammatory eicosanoids. Human cPLA2 alpha is 749 amino acids (aa) in length. It contains a phospholipid-binding and membrane-association C2 domain (aa 1-178), and an overlapping PLA2c domain that demonstrates catalytic activity (aa 140-740). While phosphorylation on Ser505 and Ser727 contribute to activation, there are at least five other utilized phosphorylation sites on the molecule. Phosphorylation may increase the MW of cPLA2 alpha in SDS-Page to about 100 kDa. Full-length (aa 1-749) human PLA2G4A/cPLA2 alpha shares 94% aa sequence identity with mouse PLA2G4A.
  • Long Name:
    Phospholipase A2 Group IV A
  • Entrez Gene IDs:
    5321 (Human); 18783 (Mouse); 24653 (Rat)
  • Alternate Names:
    calcium-dependent phospholipid-binding protein; cPLA2; cPLA2-alpha; lysophospholipase; MGC126350; phosphatidylcholine 2-acylhydrolase; Phospholipase A2 group IVA; phospholipase A2, group IVA (cytosolic, calcium-dependent); PLA2G4A; PLA2G4cytosolic phospholipase A2
Related Research Areas
Isotype Controls
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Secondary Antibodies
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Sheep IgG (H+L) PE-conjugated Antibody

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Sheep IgG (H+L) APC-conjugated Antibody

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Sheep IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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