Human/Mouse PLA2G4A Antibody Summary
Accession # NP_077734
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse PLA2G4A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse PLA2G4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6659) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLA2G4A at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PLA2G4A in Human Colon Cancer Tissue. PLA2G4A was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Sheep Anti-Human/Mouse PLA2G4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6659) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
PLA2G4A (Phospholipase A2 Group IVA; also cytosolic phospholipase A2 alpha/cPLA2 alpha and lysophospholipase) is an 100-110 kDa member of the cytosolic PLA2 family of enzymes. Expression can be induced in select cell types, including endothelium, smooth muscle, macrophages, PMNs, fibroblasts, mast cells and platelets. Tumor cells often serve as sources for cPLA2 alpha activity. cPLA2 alpha is synthesized in the cytosol where, upon activation, it translocates to sites containing cell membranes, including the Golgi, ER and nuclear membrane. This translocation allows for enzymatic action on membrane phospholipids, generating free arachidonic acid that is converted into proinflammatory eicosanoids. Human cPLA2 alpha is 749 amino acids (aa) in length. It contains a phospholipid-binding and membrane-association C2 domain (aa 1-178), and an overlapping PLA2c domain that demonstrates catalytic activity (aa 140-740). While phosphorylation on Ser505 and Ser727 contribute to activation, there are at least five other utilized phosphorylation sites on the molecule. Phosphorylation may increase the MW of cPLA2 alpha in SDS-Page to about 100 kDa. Full-length (aa 1-749) human PLA2G4A/cPLA2 alpha shares 94% aa sequence identity with mouse PLA2G4A.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human/Mouse PLA2G4A Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse PLA2G4A Antibody and earn rewards!
Have you used Human/Mouse PLA2G4A Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image