Human/Mouse/Rat Acetyl-CoA
Carboxylase alpha /ACACA Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human Acetyl-CoA Carboxylase alpha /ACACA in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Acetyl-CoA Carboxylase alpha /ACACA
    Pro1185-Phe1352
    Accession # Q13085
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat Acetyl-CoA Carboxylase alpha /ACACA by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Acetyl-CoA Carboxylase alpha /ACACA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6898) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Acetyl-CoA Carboxylase alpha /ACACA at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Acetyl-CoA Carboxylase alpha/ACACA

ACAC-A (Acetyl-CoA carboxylase alpha/1; also ACC-1 and biotin carboxylase) is a 260-265 kDa cytoplasmic, phosphorylated biotinyl-enzyme. It is widely expressed, and found to be concentrated in hepatocytes, adipocytes and lactating mammary epithelium. It is one of two gene products (ACAC-B/beta being the other) that catalyze the formation of malonyl-CoA from acetyl-CoA. The formation of malonyl-CoA by ACAC-A is a rate-limiting step in fatty acid synthesis; malonyl-CoA formed by ACAC-B acts as a regulator of CPT-1 during fatty acidoxidation. Human ACAC-A is 2346 amino acids (aa ) in length. It contains an N-terminal acetylated Met, one ATP-Grasp domain (aa 275-466) with an embedded biotin carboxylation domain (aa 117-618), a biotinyl-binding region (aa 752-818), and a carboxyltransferase domain (aa 1698-2194). There are at least 17 utilized phosphorylation sites, and two acetylated Lys. ACAC-A exists as either a dimer or higher-order oligomer. Multiple splice variants exist. One possesses an alternative start site at Met79, a second utilizes an alternative start site 37 aa upstream of the standard site, and a third (called PIII) shows a 17 aa substitution for aa 1-75. Over aa 1185-1352, human ACAC-A shares 95% aa identity with mouse ACAC-A, and 97% aa identity with both ovine and bovine ACAC-A.

  • Long Name:
    Acetyl-Coenzyme A Carboxylase alpha
  • Entrez Gene IDs:
    31 (Human); 107476 (Mouse); 60581 (Rat)
  • Alternate Names:
    ACACA; ACACacetyl-CoA carboxylase 1; ACC1; ACC1ACC; ACCA; ACC-alpha; AcetylCoA Carboxylase alpha; Acetyl-CoA Carboxylase alpha; acetyl-Coenzyme A carboxylase alpha; EC 6.4.1.2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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