Human/Mouse/Rat Actin Antibody

Catalog # Availability Size / Price Qty
AF4000
AF4000-SP
Human/Mouse/Rat Actin Antibody in Immunocytochemistry
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Product Details
Citations (5)
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Human/Mouse/Rat Actin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Actin in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Human platelet-derived Actin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Actin in HeLa Human Cell Line. View Larger

Actin in HeLa Human Cell Line.

Actin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4000) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Detection of Human/Mouse/Rat Actin by Western Blot. View Larger

Detection of Human/Mouse/Rat Actin by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4000) followed by HRP‑conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Actin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Actin in HEK293 Human Cell Line. View Larger

Actin in HEK293 Human Cell Line.

Actin was detected in immersion fixed HEK293 human embryonic kidney cell line using Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4000) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Actin

The Actin protein, a component of the eukaryotic cytoskeleton, is highly conserved between multiple species. Levels of the Actin protein may vary between different cell types, but are consistent within a given cell type, and for this reason, Actin protein levels are considered an internal control for protein loading.

Alternate Names
ACTA; Actin; actin, alpha 1, skeletal muscle; alpha skeletal muscle actin; alpha skeletal muscle; alpha-actin-1; ASMA; CFTD; CFTDM; MPFD; NEM1; NEM2; NEM3

Product Datasheets

Citations for Human/Mouse/Rat Actin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Fatty acid-binding protein 5 (FABP5) regulates cognitive function both by decreasing anandamide levels and by activating the nuclear receptor peroxisome proliferator-activated receptor beta/delta (PPARbeta/delta) in the brain.
    Authors: Yu S, Levi L, Casadesus G, Kunos G, Noy N
    J Biol Chem, 2014;289(18):12748-58.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Nuclear trafficking of the HIV-1 pre-integration complex depends on the ADAM10 intracellular domain.
    Authors: Endsley M, Somasunderam A, Li G, Oezguen N, Thiviyanathan V, Murray J, Rubin D, Hodge T, O'Brien W, Lewis B, Ferguson M
    Virology, 2014;454(0):60-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot Control
  3. Microbial exposure early in life regulates airway inflammation in mice after infection with Streptococcus pneumoniae with enhancement of local resistance.
    Authors: Yasuda Y, Matsumura Y, Kasahara K, Ouji N, Sugiura S, Mikasa K, Kita E
    Am. J. Physiol. Lung Cell Mol. Physiol., 2010;298(1):L67-78.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo.
    Authors: Siddappa R, Martens A, Doorn J, Leusink A, Olivo C, Licht R, van Rijn L, Gaspar C, Fodde R, Janssen F, van Blitterswijk C, de Boer J
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(20):7281-6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
    Authors: De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
    Biotechnol. Bioeng., 2007;97(6):1617-25.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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