Human/Mouse/Rat Actin Antibody

R&D Systems | Catalog # AF4000

R&D Systems
Discontinued Product
AF4000 has been discontinued. View all Actin products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, ELISA Capture, Western Blot Control

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Actin Primary Antibodies »

Product Specifications

Immunogen

Human platelet-derived Actin

Specificity

Detects human, mouse, and rat Actin in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Actin Antibody

Detection of Human/Mouse/Rat Actin antibody by Western Blot.

Detection of Human/Mouse/Rat Actin by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4000) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Actin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Actin antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Actin in HeLa Human Cell Line.

Actin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4000) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Actin antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

Actin in HEK293 Human Cell Line.

Actin was detected in immersion fixed HEK293 human embryonic kidney cell line using Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4000) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat Actin Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Actin

The Actin protein, a component of the eukaryotic cytoskeleton, is highly conserved between multiple species. Levels of the Actin protein may vary between different cell types, but are consistent within a given cell type, and for this reason, Actin protein levels are considered an internal control for protein loading.

Alternate Names

ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3

Additional Actin Products

Product Documents for Human/Mouse/Rat Actin Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Actin Antibody

For research use only

Citations for Human/Mouse/Rat Actin Antibody

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Protocols

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