Human/Mouse/Rat Axin-1 Antibody
Human/Mouse/Rat Axin-1 Antibody Summary
Tyr210-Glu410
Accession # O15169
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat Axin‑1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Axin-1
Axin-1 is a negative regulator of the Wnt signaling pathway, which is involved in regulation of embryonic axis formation in vertebrates. Axin-1 binds to beta -catenin and glycogen synthase kinase-3 (GSK-3) to promote beta -catenin phosphorylation and subsequent degradation. Axin-1 also binds to the adenomatous polyposis coli (APC) gene, a known tumor suppressor that also regulates beta -catenin.
Product Datasheets
Citations for Human/Mouse/Rat Axin-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Evaluation of AXIN1 and AXIN2 as targets of tankyrase inhibition in hepatocellular carcinoma cell lines
Authors: W Wang, P Liu, M Lavrijsen, S Li, R Zhang, S Li, WS van de Gee, HJG van de Wer, MP Peppelenbo, R Smits
Scientific Reports, 2021;11(1):7470.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
APC controls Wnt-induced beta-catenin destruction complex recruitment in human colonocytes
Authors: TW Parker, KL Neufeld
Sci Rep, 2020;10(1):2957.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Treatment with XAV-939 prevents in vitro calcification of human valvular interstitial cells
Authors: C Dittfeld, G Reimann, A Mieting, P Büttner, A Jannasch, K Plötze, G Steiner, SM Tugtekin, K Matschke
PLoS ONE, 2018;13(12):e0208774.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway
Authors: K Saito-Diaz, H Benchabane, A Tiwari, A Tian, B Li, JJ Thompson, AS Hyde, LM Sawyer, JN Jodoin, E Santos, LA Lee, RJ Coffey, RD Beauchamp, CS Williams, AK Kenworthy, DJ Robbins, Y Ahmed, E Lee
Dev. Cell, 2018;44(5):566-581.e8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
HSP105 recruits protein phosphatase 2A to dephosphorylate beta-catenin.
Authors: Yu N, Kakunda M, Pham V, Lill J, Du P, Wongchenko M, Yan Y, Firestein R, Huang X
Mol Cell Biol, 2015;35(8):1390-400.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Inhibition of tankyrases induces Axin stabilization and blocks Wnt signalling in breast cancer cells.
Authors: Bao R, Christova T, Song S, Angers S, Yan X, Attisano L
PLoS ONE, 2012;7(11):e48670.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Dual functions of DP1 promote biphasic Wnt-on and Wnt-off states during anteroposterior neural patterning.
EMBO J., 2012;31(16):3384-97.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Canonical Wnt signaling negatively regulates platelet function.
Authors: Steele BM, Harper MT, Macaulay IC, Morrell CN, Perez-Tamayo A, Foy M, Habas R, Poole AW, Fitzgerald DJ, Maguire PB
Proc. Natl. Acad. Sci. U.S.A., 2009;106(47):19836-41.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling.
Authors: Lebensohn A, Dubey R, Neitzel L, Tacchelly-Benites O, Yang E, Marceau C, Davis E, Patel B, Bahrami-Nejad Z, Travaglini K, Ahmed Y, Lee E, Carette J, Rohatgi R
Elife, 0;5(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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From left to right are lanes: negative control without or with Wnt3a treatment, and Axin1 stabilizer XAV939 without or with Wnt3a treatment.
Specificity: Specific
Sensitivity: Sensitive
Buffer: TBS+Tween 0.1%
Dilution: 1/1000
