Human/Mouse/Rat Axin-1 Antibody

(4 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat Axin-1 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Axin-1
    Tyr210-Glu410
    Accession # O15169
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat Axin‑1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Axin-1

Axin-1 is a negative regulator of the Wnt signaling pathway, which is involved in regulation of embryonic axis formation in vertebrates. Axin-1 binds to beta -catenin and glycogen synthase kinase-3 (GSK-3) to promote beta -catenin phosphorylation and subsequent degradation. Axin-1 also binds to the adenomatous polyposis coli (APC) gene, a known tumor suppressor that also regulates beta -catenin.

  • Long Name:
    Axis Inhibitor 1
  • Entrez Gene IDs:
    8312 (Human); 12005 (Mouse); 79257 (Rat)
  • Alternate Names:
    axin 1; Axin1; Axin-1; AXINaxis inhibitor 1; Axis inhibition protein 1; fused, mouse, homolog of; hAxin; MGC52315
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. HSP105 recruits protein phosphatase 2A to dephosphorylate beta-catenin.
    Authors: Yu N, Kakunda M, Pham V, Lill J, Du P, Wongchenko M, Yan Y, Firestein R, Huang X
    Mol Cell Biol, 2015;35(8):1390-400.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  2. Dual functions of DP1 promote biphasic Wnt-on and Wnt-off states during anteroposterior neural patterning.
    EMBO J., 2012;31(16):3384-97.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Inhibition of tankyrases induces Axin stabilization and blocks Wnt signalling in breast cancer cells.
    Authors: Bao R, Christova T, Song S, Angers S, Yan X, Attisano L
    PLoS ONE, 2012;7(11):e48670.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Canonical Wnt signaling negatively regulates platelet function.
    Authors: Steele BM, Harper MT, Macaulay IC, Morrell CN, Perez-Tamayo A, Foy M, Habas R, Poole AW, Fitzgerald DJ, Maguire PB
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(47):19836-41.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human/Mouse/Rat Axin‑1 Antibody AF3287
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 WB Mouse Anonymous 10/26/2015
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Western Blot Human/Mouse/Rat Axin‑1 Antibody AF3287
Western Blot: Human/Mouse/Rat Axin‑1 Antibody [AF3287]

Summary

ApplicationWestern Blot
Sample TestedEMT6 cells
SpeciesMouse

Other Experimental Details

Other Experimental DetailsFrom left to right are lanes: negative control without or with Wnt3a treatment, and Axin1 stabilizer XAV939 without or with Wnt3a treatment.
Specificity: Specific
Sensitivity: Sensitive
Buffer: TBS+Tween 0.1%
Dilution: 1/1000

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