Human/Mouse/Rat Axin-1 Antibody

Catalog # Availability Size / Price Qty
AF3287
AF3287-SP
Detection of Human/Mouse/Rat Axin‑1 by Western Blot.
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Product Details
Citations (9)
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Reviews (1)

Human/Mouse/Rat Axin-1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat Axin-1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Axin-1
Tyr210-Glu410
Accession # O15169
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Axin‑1 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Axin‑1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axin-1

Axin-1 is a negative regulator of the Wnt signaling pathway, which is involved in regulation of embryonic axis formation in vertebrates. Axin-1 binds to beta -catenin and glycogen synthase kinase-3 (GSK-3) to promote beta -catenin phosphorylation and subsequent degradation. Axin-1 also binds to the adenomatous polyposis coli (APC) gene, a known tumor suppressor that also regulates beta -catenin.

Long Name
Axis Inhibitor 1
Entrez Gene IDs
8312 (Human); 12005 (Mouse); 79257 (Rat)
Alternate Names
axin 1; Axin1; Axin-1; AXINaxis inhibitor 1; Axis inhibition protein 1; fused, mouse, homolog of; hAxin; MGC52315

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Citations for Human/Mouse/Rat Axin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Evaluation of AXIN1 and AXIN2 as targets of tankyrase inhibition in hepatocellular carcinoma cell lines
    Authors: W Wang, P Liu, M Lavrijsen, S Li, R Zhang, S Li, WS van de Gee, HJG van de Wer, MP Peppelenbo, R Smits
    Scientific Reports, 2021;11(1):7470.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. APC controls Wnt-induced beta-catenin destruction complex recruitment in human colonocytes
    Authors: TW Parker, KL Neufeld
    Sci Rep, 2020;10(1):2957.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Treatment with XAV-939 prevents in vitro calcification of human valvular interstitial cells
    Authors: C Dittfeld, G Reimann, A Mieting, P Büttner, A Jannasch, K Plötze, G Steiner, SM Tugtekin, K Matschke
    PLoS ONE, 2018;13(12):e0208774.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway
    Authors: K Saito-Diaz, H Benchabane, A Tiwari, A Tian, B Li, JJ Thompson, AS Hyde, LM Sawyer, JN Jodoin, E Santos, LA Lee, RJ Coffey, RD Beauchamp, CS Williams, AK Kenworthy, DJ Robbins, Y Ahmed, E Lee
    Dev. Cell, 2018;44(5):566-581.e8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. HSP105 recruits protein phosphatase 2A to dephosphorylate beta-catenin.
    Authors: Yu N, Kakunda M, Pham V, Lill J, Du P, Wongchenko M, Yan Y, Firestein R, Huang X
    Mol Cell Biol, 2015;35(8):1390-400.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  6. Inhibition of tankyrases induces Axin stabilization and blocks Wnt signalling in breast cancer cells.
    Authors: Bao R, Christova T, Song S, Angers S, Yan X, Attisano L
    PLoS ONE, 2012;7(11):e48670.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Dual functions of DP1 promote biphasic Wnt-on and Wnt-off states during anteroposterior neural patterning.
    EMBO J., 2012;31(16):3384-97.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Canonical Wnt signaling negatively regulates platelet function.
    Authors: Steele BM, Harper MT, Macaulay IC, Morrell CN, Perez-Tamayo A, Foy M, Habas R, Poole AW, Fitzgerald DJ, Maguire PB
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(47):19836-41.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling.
    Authors: Lebensohn A, Dubey R, Neitzel L, Tacchelly-Benites O, Yang E, Marceau C, Davis E, Patel B, Bahrami-Nejad Z, Travaglini K, Ahmed Y, Lee E, Carette J, Rohatgi R
    Elife, 0;5(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat Axin-1 Antibody

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Human/Mouse/Rat Axin‑1 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: EMT6 cells Species: Mouse

From left to right are lanes: negative control without or with Wnt3a treatment, and Axin1 stabilizer XAV939 without or with Wnt3a treatment.
Specificity: Specific
Sensitivity: Sensitive
Buffer: TBS+Tween 0.1%
Dilution: 1/1000