Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Caveolin-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Caveolin-2
Met1-Lys86
Accession # P51636

Specificity

Caveolin-2 antibodies are ideal for immunocytochemistry colocalization studies in caveolae. The unconjugated antibody detects endogenous human, mouse, and rat Caveolin-2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Caveolin‑2 Biotinylated Antibody

Caveolin-2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Caveolin‑2 in HeLa Human Cell Line.

Caveolin-2 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Caveolin-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5788) at 5 µg/mL overnight at 4° C. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to caveolae.

Caveolin‑2 in L6 rat myoblast cell line, A549 human lung carcinoma cell line and C2C12 mouse myoblast cell line.

Caveolin‑2 was detected in immersion fixed L6 rat myoblast cell line, A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line using Goat Anti-Human/Mouse/Rat Caveolin‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5788) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Applications for Human/Mouse/Rat Caveolin‑2 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Formaldehyde fixed HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Caveolin-2

Caveolin-2 is a cell membrane-associated member of the caveolin family of proteins. Although its predicted MW is 17 kDa, it runs anomalously at 21‑22 kDa on SDS‑PAGE. Caveolin-2 is not ubiquitous, but it is found in divergent cell types such as smooth muscle, endothelial, and skeletal muscle cells, plus fibroblasts and adipocytes. When expressed alone, Caveolin-2 forms monomers and homodimers; when expressed with Caveolin-1, it exists as part of a 150 kDa, Caveolin-1 associated oligomeric complex. Human Caveolin-2 is 162 amino acids (aa) in length. It contains a central membrane-embedded sequence (aa 87‑107) flanked by two cytoplasmic domains (aa 1‑86 and 108‑162). Caveolin-2 has been found phosphorylated at tyrosines 19 and 27, and at serines 20, 23 and 36.

Alternate Names

CAV2, Caveolin2

Entrez Gene IDs

858 (Human); 12390 (Mouse); 363425 (Rat)

Gene Symbol

CAV2

UniProt

P51636

Additional Caveolin-2 Products

Product Documents for Human/Mouse/Rat Caveolin‑2 Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Caveolin‑2 Biotinylated Antibody

For research use only

Related Research Areas

Cancer Biomarkers

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