Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Caveolin-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Caveolin-2
Met1-Lys86
Accession # P51636

Specificity

Detects endogenous human, mouse, and rat Caveolin-2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Caveolin‑2 Antibody

Detection of Human Caveolin‑2 antibody by Western Blot.

Detection of Human Caveolin‑2 by Western Blot.

Western blot shows recombinant human Caveolin-1, Caveolin-2 and Caveolin-3 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat Caveolin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band for Caveolin-2 was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Caveolin‑2 antibody in Human K562 Cells by Immunocytochemistry (ICC).

Caveolin‑2 in Human K562 Cells.

Caveolin-2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using 10 µg/mL Goat Anti-Human/Mouse/Rat Caveolin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue).

Caveolin‑2 antibody in Human Liver by Immunohistochemistry (IHC-P).

Caveolin‑2 in Human Liver.

Caveolin-2 was detected in paraffin-embedded sections of human liver using Goat Anti-Human/Mouse/Rat Caveolin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human/Mouse/Rat Caveolin‑2 antibody by Western Blot.

Detection of Human/Mouse/Rat Caveolin‑2 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat Caveolin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Caveolin-2 was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Caveolin-2 in L6 rat myoblast cell line, A549 human lung carcinoma cell line and C2C12 mouse myoblast cell line.

Caveolin‑2 was detected in immersion fixed L6 rat myoblast cell line, A549 human lung carcinoma cell line and C2C12 mouse myoblast cell line using Goat Anti-Human/Mouse/Rat Caveolin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Applications for Human/Mouse/Rat Caveolin‑2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

1 µg/mL
Sample: A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Caveolin-2

Caveolin-2 is a cell membrane-associated member of the caveolin family of proteins. Although its predicted MW is 17 kDa, it runs anomalously at 21‑22 kDa on SDS‑PAGE. Caveolin-2 is not ubiquitous, but it is found in divergent cell types such as smooth muscle, endothelial, and skeletal muscle cells, plus fibroblasts and adipocytes. When expressed alone, Caveolin-2 forms monomers and homodimers; when expressed with Caveolin-1, it exists as part of a 150 kDa, Caveolin-1 associated oligomeric complex. Human Caveolin-2 is 162 amino acids (aa) in length. It contains a central membrane-embedded sequence (aa 87‑107) flanked by two cytoplasmic domains (aa 1‑86 and 108‑162). Caveolin-2 has been found phosphorylated at tyrosines 19 and 27, and at serines 20, 23 and 36.

Alternate Names

CAV2, Caveolin2

Entrez Gene IDs

858 (Human); 12390 (Mouse); 363425 (Rat)

Gene Symbol

CAV2

UniProt

P51636

Additional Caveolin-2 Products

Product Documents for Human/Mouse/Rat Caveolin‑2 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Caveolin‑2 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human/Mouse/Rat Caveolin‑2 Antibody

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Protocols

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