Human/Mouse/Rat Caveolin-2 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects endogenous human, mouse, and rat Caveolin-2 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Caveolin-2
    Met1-Lys86
    Accession # P51636
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Caveolin‑2 in Human K562 Cells. Caveolin‑2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using 10 µg/mL Goat Anti-Human/Mouse/Rat Caveolin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
Caveolin‑2 in Human Liver. Caveolin‑2 was detected in paraffin-embedded sections of human liver using Goat Anti-Human/Mouse/Rat Caveolin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) at 10 µg/mL overnight at
4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human/Mouse/Rat Caveolin‑2 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat Caveolin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Caveolin‑2 was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Caveolin‑2 by Western Blot. Western blot shows recombinant human Caveolin-1, Caveolin-2 and Caveolin-3 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat Caveolin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Caveolin‑2 was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caveolin-2
Caveolin-2 is a cell membrane-associated member of the caveolin family of proteins. Although its predicted MW is 17 kDa, it runs anomalously at 21‑22 kDa on SDS‑PAGE. Caveolin-2 is not ubiquitous, but it is found in divergent cell types such as smooth muscle, endothelial, and skeletal muscle cells, plus fibroblasts and adipocytes. When expressed alone, Caveolin-2 forms monomers and homodimers; when expressed with Caveolin-1, it exists as part of a 150 kDa, Caveolin-1 associated oligomeric complex. Human Caveolin-2 is 162 amino acids (aa) in length. It contains a central membrane-embedded sequence (aa 87‑107) flanked by two cytoplasmic domains (aa 1‑86 and 108‑162). Caveolin-2 has been found phosphorylated at tyrosines 19 and 27, and at serines 20, 23 and 36.
  • Entrez Gene IDs:
    858 (Human); 12390 (Mouse); 363425 (Rat)
  • Alternate Names:
    CAV; CAV2; caveolae protein, 20-kD; caveolin 2 isoform a and b; caveolin 2; Caveolin2; Caveolin-2; MGC12294
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