Human/Mouse/Rat CBP Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat CBP by Western Blot.
2 Images
Product Details
Citations (2)
Supplemental Products

Human/Mouse/Rat CBP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse and rat CBP.
Monoclonal Mouse IgG2B Clone # 257003
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human CBP
Accession # Q92793
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
8-25 µg/mL
Immersion fixed HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat CBP antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat CBP by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat CBP Monoclonal Antibody (Catalog # MAB2676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CBP at approximately 265 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry View Larger

CBP in HeLa Human Cell Line. CBP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat CBP Monoclonal Antibody (Catalog # MAB2676) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CBP

The CBP (CREB-binding protein) is a histone acetyltransferase and transcriptional coactivator that regulates RNA polymerase II transcription. In addition to histone acetylation, CBP also acetylates various transcription factors, including the tumor suppressor protein p53, to enhance sequence-specific DNA binding.

Long Name
CREB-binding Protein
Entrez Gene IDs
1387 (Human); 12914 (Mouse); 54244 (Rat)
Alternate Names
CBP; CBPRTS; CREB binding protein; CREB-binding protein; CREBBP; EC; KAT3A; RSTS; RTS; Rubinstein-Taybi syndrome

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Citations for Human/Mouse/Rat CBP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Respiratory syncytial virus impairs macrophage IFN-alpha/beta- and IFN-gamma-stimulated transcription by distinct mechanisms.
    Authors: Senft AP, Taylor RH, Lei W, Campbell SA, Tipper JL, Martinez MJ, Witt TL, Clay CC, Harrod KS
    Am. J. Respir. Cell Mol. Biol., 2010;42(4):404-14.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Effect of cAMP on TGFbeta1-induced corneal keratocyte-myofibroblast transformation.
    Authors: Xing D, Bonanno JA
    Invest. Ophthalmol. Vis. Sci., 2009;50(2):626-33.
    Species: Rabbit
    Sample Types: Cell Lysates
    Applications: Co-Immunoprecipitation


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Reconstitution Buffers

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