Human/Mouse/Rat CDC25C Antibody Summary
Accession # P30307
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse CDC25C by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat CDC25C Monoclonal Antibody (Catalog # MAB4459) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CDC25C at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cell Division Cycle 25C (CDC25C) is a dual serine/threonine and tyrosine phosphatase. There are four splice variants with molecular weights of 46, 47, 49, and 53 kDa. The smaller variants lack key regulatory sites and their upregulation has been observed in prostate cancer. CDC25C regulates the G2/M cell cycle transition by dephosphorylating proteins such as Chk1. Its cellular distribution is controlled by phosphorylations that enhance 14-3-3 binding, block nuclear localization, and inhibit enzymatic activity.
Citation for Human/Mouse/Rat CDC25C Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
BCL9 promotes tumor progression by conferring enhanced proliferative, metastatic, and angiogenic properties to cancer cells.
Authors: Mani M, Carrasco DE, Zhang Y, Takada K, Gatt ME, Dutta-Simmons J, Ikeda H, Diaz-Griffero F, Pena-Cruz V, Bertagnolli M, Myeroff LL, Markowitz SD, Anderson KC, Carrasco DR
Cancer Res., 2009;69(19):7577-86.
Sample Types: Whole Tissue
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