Human/Mouse/Rat Contactin-2/TAG1 Antibody Summary
Accession # Q02246
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Contactin‑2/TAG1 in Human Brain. Contactin‑2/TAG1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse/Rat Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1714) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human, Mouse, and Rat Contactin‑2/TAG1 by Western Blot. Western blot shows lysates of human cerebellum tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1714) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Contactin‑2/TAG1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Contactin-2 (CNTN2), also called TAG-1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl‑phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1‑3). Human Contactin-2 cDNA encodes a 28 amino acid (aa) signal peptide, a 984 aa mature secreted protein with six Ig-like domains followed by four fibronectin type III‑like repeats, and a 28 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature human Contactin-2 shares approximately 93%, 93% and 75% aa sequence identity with human, rat and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3‑5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3‑6). In mature myelinated fibers, Contactin‑2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits caspr2 (Contactin‑associated protein 2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7‑10). The axonal Contactin‑2 interacts in cis with caspr2, and in trans with another Contactin‑2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).
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- Karagogeos, D. (2003) Front. Biosci.8:s1304.
- Liu, Y. & M.C. Halloran (2005) J. Neurosci. 25:10556.
- Denaxa, M. et al. (2005) Dev. Biol. 288:87.
- Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
- Traka, M. et al. (2002) J. Neurosci. 22:3016.
- Poliak, S. and E. Peles (2003) Nature Reviews Neurosci. 4:968.
- Traka, M. et al. (2003) J. Cell Biol. 162:1161.
- Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
Citations for Human/Mouse/Rat Contactin-2/TAG1 Antibody
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Citations: Showing 1 - 4
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Deletion of Nkx2-5 in trabecular myocardium reveals the developmental origins of pathological heterogeneity associated with ventricular non-compaction cardiomyopathy
Authors: C Choquet, THM Nguyen, P Sicard, E Buttigieg, TT Tran, F Kober, I Varlet, R Sturny, MW Costa, RP Harvey, C Nguyen, P Rihet, S Richard, M Bernard, RG Kelly, N Lalevée, L Miquerol
PLoS Genet., 2018;14(7):e1007502.
Sample Types: Whole Tissue
hPSC Modeling Reveals that Fate Selection of Cortical Deep Projection Neurons Occurs in the Subplate
Authors: MZ Ozair, C Kirst, BL van den Be, A Ruzo, T Rito, AH Brivanlou
Cell Stem Cell, 2018;0(0):.
Sample Types: Whole Tissue
Genetic analysis of the organization, development and plasticity of corneal innervation in mice
Authors: N Bouheraoua, S Fouquet, M Teresa Mar, D Karagogeos, L Laroche, A Chédotal
J. Neurosci., 2018;0(0):.
Sample Types: Whole Tissue
Applications: 3D Microscopy
Lewis(x) and alpha2,3-sialyl glycans and their receptors TAG-1, Contactin, and L1 mediate CD24-dependent neurite outgrowth.
Authors: Lieberoth A, Splittstoesser F, Katagihallimath N, Jakovcevski I, Loers G, Ranscht B, Karagogeos D, Schachner M, Kleene R
J. Neurosci., 2009;29(20):6677-90.
Sample Types: Tissue Homogenates
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