Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DARPP-32 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DARPP‑32
Arg51-Ala204
Accession # Q9UD71

Specificity

Detects human, mouse, and rat DARPP‑32 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat DARPP‑32 Antibody

Detection of Human, Mouse, and Rat DARPP-32 antibody by Western Blot.

Detection of Human, Mouse, and Rat DARPP‑32 by Western Blot.

Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DARPP-32 at approximately 32 - 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DARPP-32 antibody in Human Brain by Immunohistochemistry (IHC-P).

DARPP‑32 in Human Brain.

DARPP-32 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
DARPP-32 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

DARPP‑32 in Mouse Brain.

DARPP-32 was detected in perfusion fixed frozen sections of mouse brain (caudate putamen) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
DARPP-32 antibody in Rat Brain by Immunohistochemistry (IHC-Fr).

DARPP‑32 in Rat Brain.

DARPP-32 was detected in perfusion fixed frozen sections of rat brain (globus pallidus) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse/Rat DARPP‑32 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus), and perfusion fixed frozen sections of mouse brain (caudate putamen) and rat brain (globus pallidus) 

Western Blot

1 µg/mL
Sample: Human brain tissue, mouse brain tissue, and rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DARPP-32

Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kDa protein that anomalously migrates at about 32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP‑32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.

Long Name

Dopamine and cAMP-regulated Phosphoprotein

Alternate Names

DARPP32, PPP1R1B

Entrez Gene IDs

84152 (Human); 19049 (Mouse); 360616 (Rat)

Gene Symbol

PPP1R1B

UniProt

Q9UD71 (Human)

Additional DARPP-32 Products

Product Documents for Human/Mouse/Rat DARPP‑32 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat DARPP‑32 Antibody

For research use only

Related Research Areas

Phosphatases and Regulators

Citations for Human/Mouse/Rat DARPP‑32 Antibody

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Protocols

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