Human/Mouse/Rat DOK7 Antibody Summary
Accession # Q18PE1
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat DOK7 by Western Blot. Western blot shows lysates of untreated MCF-7 human breast cancer cell line and T47D human breast cancer cell line and C2C12 mouse myoblast cell line and L6 rat myoblast cell line untreated (-) or treated (+) with 2% horse serum for 6 days. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat DOK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DOK7 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human DOK7 by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for DOK7 at approximately 60 & 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat DOK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
DOK7 (Downstream of kinase 7) is a 55 kDa member of the DOK family of cytoplasmic adaptor proteins. It links the acetylcholine receptor and the receptor tyrosine kinase MuSK in skeletal and cardiac muscle. Mutations can cause familial myasthenic syndromes. The 504 amino acid (aa) human DOK7 contains pleckstrin homology (aa 7‑109) and phosphotyrosine-binding (PTK, aa 125‑236) and SH2 domains and a C‑terminal nuclear export signal. Splicing isoforms of 255, 608, and 366 aa diverge at aa 175 or 500, or have 40 divergent aa replacing aa 1‑178, respectively. Within aa 179‑299, human DOK7 shares 92% and 93% aa identity with mouse and rat DOK7, respectively.
Citations for Human/Mouse/Rat DOK7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Sarcoglycan Alpha Mitigates Neuromuscular Junction Decline in Aged Mice by Stabilizing LRP4
Authors: K Zhao, C Shen, L Li, H Wu, G Xing, Z Dong, H Jing, W Chen, H Zhang, Z Tan, J Pan, L Xiong, H Wang, W Cui, XD Sun, S Li, X Huang, WC Xiong, L Mei
J. Neurosci., 2018;0(0):.
Sample Types: Cell Lysates
Applications: Western Blot
Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation.
Authors: Hallock P, Chin S, Blais S, Neubert T, Glass D
Mol Cell Biol, 2015;36(2):262-70.
Sample Types: Cell Lysates
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