Human/Mouse/Rat DOK7 Antibody

Catalog # Availability Size / Price Qty
AF6398
AF6398-SP
Human/Mouse/Rat DOK7 Antibody in Western Blot
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human/Mouse/Rat DOK7 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat DOK7 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human DOK7
Ala179-Pro299
Accession # Q18PE1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat DOK7 by Western Blot. View Larger

Detection of Human, Mouse, and Rat DOK7 by Western Blot. Western blot shows lysates of untreated MCF-7 human breast cancer cell line and T47D human breast cancer cell line and C2C12 mouse myoblast cell line and L6 rat myoblast cell line untreated (-) or treated (+) with 2% horse serum for 6 days. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat DOK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DOK7 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human DOK7 by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human DOK7 by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for DOK7 at approximately 60 & 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat DOK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DOK7

DOK7 (Downstream of kinase 7) is a 55 kDa member of the DOK family of cytoplasmic adaptor proteins. It links the acetylcholine receptor and the receptor tyrosine kinase MuSK in skeletal and cardiac muscle. Mutations can cause familial myasthenic syndromes. The 504 amino acid (aa) human DOK7 contains pleckstrin homology (aa 7‑109) and phosphotyrosine-binding (PTK, aa 125‑236) and SH2 domains and a C‑terminal nuclear export signal. Splicing isoforms of 255, 608, and 366 aa diverge at aa 175 or 500, or have 40 divergent aa replacing aa 1‑178, respectively. Within aa 179‑299, human DOK7 shares 92% and 93% aa identity with mouse and rat DOK7, respectively.

Long Name
Downstream of Tyrosine Kinase 7
Entrez Gene IDs
285489 (Human); 231134 (Mouse); 305448 (Rat)
Alternate Names
C4orf25; C4orf25chromosome 4 open reading frame 25; CMS1B; docking protein 7; DOK7; Dok-7; Downstream of tyrosine kinase 7; FLJ33718; FLJ39137; FLJ90556; protein Dok-7

Product Datasheets

Citations for Human/Mouse/Rat DOK7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Sarcoglycan Alpha Mitigates Neuromuscular Junction Decline in Aged Mice by Stabilizing LRP4
    Authors: K Zhao, C Shen, L Li, H Wu, G Xing, Z Dong, H Jing, W Chen, H Zhang, Z Tan, J Pan, L Xiong, H Wang, W Cui, XD Sun, S Li, X Huang, WC Xiong, L Mei
    J. Neurosci., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation.
    Authors: Hallock P, Chin S, Blais S, Neubert T, Glass D
    Mol Cell Biol, 2015;36(2):262-70.
    Species: Mouse
    Sample Types: Cell Lysates

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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