DYRK2 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 2) is a member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. DYRK2 is expressed in multiple tissues and shows dual substrate specificity. Autophosphorylation on Tyr382 leads to self-activation, while target proteins are Ser/Thr phosphorylated. Targets include NFATc, glycogen synthase, and p53. p53 phosphorylation on Ser46 by DYRK2 leads to cell apoptosis in response to DNA damage. Human DYRK2 is 601 amino acids (aa) in length, and contains one kinase catalytic domain (aa 222‑535).
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human DYRK2
Met74-Glu180
Accession # Q92630
Met74-Glu180
Accession # Q92630
Specificity
Detects endogenous human, mouse and rat DYRK2 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat DYRK2 Antibody
Detection of Human/Mouse/Rat DYRK2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat DYRK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5408) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for DYRK2 at approximately 58 to 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human/Mouse/Rat DYRK2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: DYRK2
Long Name
Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 2
Alternate Names
dual specificity tyrosine-phosphorylation-regulated kinase 2, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2, EC 2.7.12, EC 2.7.12.1, FLJ21217, FLJ21365
Gene Symbol
DYRK2
UniProt
Additional DYRK2 Products
Product Documents for Human/Mouse/Rat DYRK2 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse/Rat DYRK2 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars