Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all DYRK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DYRK2
Met74-Glu180
Accession # Q92630

Specificity

Detects endogenous human, mouse and rat DYRK2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat DYRK2 Antibody

Detection of Human/Mouse/Rat DYRK2 antibody by Western Blot.

Detection of Human/Mouse/Rat DYRK2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat DYRK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5408) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for DYRK2 at approximately 58 to 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat DYRK2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DYRK2

DYRK2 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 2) is a member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. DYRK2 is expressed in multiple tissues and shows dual substrate specificity. Autophosphorylation on Tyr382 leads to self-activation, while target proteins are Ser/Thr phosphorylated. Targets include NFATc, glycogen synthase, and p53. p53 phosphorylation on Ser46 by DYRK2 leads to cell apoptosis in response to DNA damage. Human DYRK2 is 601 amino acids (aa) in length, and contains one kinase catalytic domain (aa 222‑535).

Long Name

Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 2

Alternate Names

dual specificity tyrosine-phosphorylation-regulated kinase 2, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2, EC 2.7.12, EC 2.7.12.1, FLJ21217, FLJ21365

Entrez Gene IDs

8445 (Human); 69181 (Mouse); 314862 (Rat)

Gene Symbol

DYRK2

UniProt

Q92630

Additional DYRK2 Products

Product Documents for Human/Mouse/Rat DYRK2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat DYRK2 Antibody

For research use only

Related Research Areas

Intracellular Kinases

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