Human/Mouse/Rat DYRK2 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects endogenous human, mouse and rat DYRK2 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human DYRK2
    Met74-Glu180
    Accession # Q92630
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat DYRK2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, BaF3 mouse pro‑B cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat DYRK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5408) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for DYRK2 at approximately 58 to 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DYRK2

DYRK2 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 2) is a member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. DYRK2 is expressed in multiple tissues and shows dual substrate specificity. Autophosphorylation on Tyr382 leads to self-activation, while target proteins are Ser/Thr phosphorylated. Targets include NFATc, glycogen synthase, and p53. p53 phosphorylation on Ser46 by DYRK2 leads to cell apoptosis in response to DNA damage. Human DYRK2 is 601 amino acids (aa) in length, and contains one kinase catalytic domain (aa 222‑535).

  • Long Name:
    Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 2
  • Entrez Gene IDs:
    8445 (Human); 69181 (Mouse); 314862 (Rat)
  • Alternate Names:
    dual specificity tyrosine-phosphorylation-regulated kinase 2; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2; DYRK2; EC 2.7.12; EC 2.7.12.1; FLJ21217; FLJ21365
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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