Human/Mouse/Rat ERK1/ERK2 Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB1576G Datasheet / COA / SDS

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FAB1576G-100UG

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All ERK1/ERK2 Antibodies

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Human/Mouse/Rat ERK1/ERK2 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat ERK1/ERK2 in Western blots.

Source

Monoclonal Mouse IgG1 Clone # 216703

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human ERK1
Met1-Pro379
Accession # P27361

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activates both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. The two proteins share 83% amino acid identity, differing mainly at the N and C termini.

Long Name

Extracellular Signal-regulated Kinase 1/2

Alternate Names

ERK1; ERK-1; ERK1/ERK2; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAPK 1; Microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; p44erk1; p44-ERK1; p44mapk; p44-MAPK; PRKM3