Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Simple Western, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 216703
View all ERK1/ERK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ERK1
Met1-Pro379
Accession # P27361

Specificity

Detects human, mouse, and rat ERK1/ERK2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat ERK1/ERK2 Antibody

Detection of Human/Mouse/Rat ERK1/ERK2 antibody by Western Blot.

Detection of Human/Mouse/Rat ERK1/ERK2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human ERK1 and ERK2 (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44/42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
ERK1/ERK2 antibody in Human Brain by Immunohistochemistry (IHC-P).

ERK1/ERK2 in Human Brain.

ERK1/ERK2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse/Rat ERK1/ERK2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain cortex

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and TS1 mouse helper T cell line

Reviewed Applications

Read 8 reviews rated 4 using MAB1576 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activates both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. The two proteins share 83% amino acid identity, differing mainly at the N and C termini.

Long Name

Extracellular Signal-regulated Kinase 1/2

Alternate Names

ERK1, ERK-1, ERT2, Extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAPK 1, Microtubule-associated protein 2 kinase, mitogen-activated protein kinase 3, p44erk1, p44-ERK1, p44mapk, p44-MAPK, PRKM3

UniProt

P27361

Additional ERK1/ERK2 Products

Product Documents for Human/Mouse/Rat ERK1/ERK2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat ERK1/ERK2 Antibody

For research use only

Citations for Human/Mouse/Rat ERK1/ERK2 Antibody

Customer Reviews for Human/Mouse/Rat ERK1/ERK2 Antibody (8)

4 out of 5
8 Customer Ratings
5 Stars
13%
4 Stars
75%
3 Stars
13%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 8 reviews Showing All
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  • Human/Mouse/Rat ERK1/ERK2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Mammary tissue
    Species: Human
    Verified Customer | Posted 09/08/2021
    Human/Mouse/Rat ERK1/ERK2 Antibody MAB1576
  • Human/Mouse/Rat ERK1/ERK2 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human/Mouse/Rat ERK1/ERK2 Antibody MAB1576
  • Human/Mouse/Rat ERK1/ERK2 Antibody
    Name: Carly Garrison
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/20/2018
  • Human/Mouse/Rat ERK1/ERK2 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018
  • Human/Mouse/Rat ERK1/ERK2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/11/2018
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22818713
    Species: Rat
    Verified Customer | Posted 02/10/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 25255977
    Species: Human
    Verified Customer | Posted 02/10/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 24888374
    Species: Human
    Verified Customer | Posted 02/10/2015

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Protocols

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