Human/Mouse/Rat ERK1/ERK2 Antibody
Human/Mouse/Rat ERK1/ERK2 Antibody Summary
Met1-Pro379
Accession # P27361
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat ERK1/ERK2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human ERK1 and ERK2 (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44/42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
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ERK1/ERK2 in Human Brain. ERK1/ERK2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ERK1/ERK2
ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activates both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. The two proteins share 83% amino acid identity, differing mainly at the N and C termini.
Product Datasheets
Citations for Human/Mouse/Rat ERK1/ERK2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Combined anticancer therapy with imidazoacridinone C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesis
Authors: M ?witalska, B Filip-Psur, M Milczarek, M Psurski, A Moszy?ska, AM D?browska, M Gawro?ska, K Krzymi?ski, M Bagi?ski, R Bartoszews, J Wietrzyk
Oncogene, 2022;0(0):.
Species: Xenograft
Sample Types: Tissue Homogenates
Applications: Capillary Immunoblot -
Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning
Authors: E Soleimanpo, JR Bergado Ac, P Landgraf, D Mayer, E Dankert, DC Dieterich, M Fendt
Cells, 2021;10(2):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Safety and Activity of the Combination of Ceritinib and Dasatinib in Osteosarcoma
Authors: O Beck, C Paret, A Russo, J Burhenne, M Fresnais, K Steimel, L Seidmann, DC Wagner, N Vewinger, N Lehmann, M Sprang, N Backes, L Roth, MA Neu, A Wingerter, N Henninger, KE Malki, H Otto, F Alt, A Desuki, T Kindler, J Faber
Cancers (Basel), 2020;12(4):.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Sirtuin 3 (SIRT3) Regulates ?-Smooth Muscle Actin (?-SMA) Production through the Succinate Dehydrogenase-G Protein-coupled Receptor 91 (GPR91) Pathway in Hepatic Stellate Cells
J Biol Chem, 2016;291(19):10277-92.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The carotenoid lutein enhances matrix metalloproteinase-9 production and phagocytosis through intracellular ROS generation and ERK1/2, p38 MAPK, and RARbeta activation in murine macrophages.
Authors: Lo H, Chen C, Yang C, Wu P, Tsou C, Chiang K, Wu W
J Leukoc Biol, 2013;93(5):723-35.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Perlecan Domain V induces VEGF secretion in brain endothelial cells through integrin alpha5beta1 and ERK-dependent signaling pathways.
Authors: Clarke D, Al Ahmad A, Lee B, Parham C, Auckland L, Fertala A, Kahle M, Shaw C, Roberts J, Bix G
PLoS ONE, 2012;7(9):e45257.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation.
Authors: Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C
J. Mol. Cell. Cardiol., 2010;48(6):1225-35.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages.
Authors: Lane AE, Tan JT, Hawkins CL
Biochem. J., 2010;430(1):161-9.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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