Human/Mouse/Rat FoxP3 PE‑conjugated Antibody

R&D Systems | Catalog # IC8970P

Recombinant Monoclonal Antibody.
R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 376209
View all FoxP3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FoxP3
Gln105-Lys200
Accession # Q9BZS1

Specificity

Detects human FoxP3 in direct ELISAs. Detects human, mouse, and rat FoxP3 in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat FoxP3 PE‑conjugated Antibody

Detection of FoxP3 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of FoxP3 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Mouse/Rat FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8970P) and Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of FoxP3 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of FoxP3 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Mouse/Rat FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8970P) and Mouse Anti-Human CD25/IL-2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1020A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of FoxP3 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Mouse Anti-Human/Mouse/Rat FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8970P) and Rat Anti-Mouse CD4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB554G). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of FoxP3 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Mouse Anti-Human/Mouse/Rat FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8970P) and Rat Anti-Mouse CD25/IL-2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB2438A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of FoxP3 antibody in Rat Splenocytes antibody by Flow Cytometry.

Detection of FoxP3 in Rat Splenocytes by Flow Cytometry.

Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8970P) and Anti-Rat CD4 APC-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse/Rat FoxP3 PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs), mouse splenocytes, and rat splenocytes fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FoxP3

Human FoxP3 is a 431 amino acid (aa), 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three alternative splicing events have been described in human. The first shows a deletion of aa 72-106, a second exhibits a deletion of aa 246-272, and a third records a 60 aa insertion after Lys382. These events are likely not to be mutually exclusive. The first isoform (plus the full-length form) occurs in CD4+CD25+ T cells, while the less frequent second isoform appears in CD8+ Tregs. The mouse FoxP3 gene does not appear to be spliced. In addition, FoxP3 is found only in Tregs in mouse, while human FoxP3 appears in both Tregs and CD4+ and CD8+ conventional T cells.

Both mouse and human FoxP3 undergo proteolytic processing, after Arg51 and Arg417 in human, and at equivalent sites in mouse. Cleavage at the N-terminus generate a 41 kDa isoform, whereas C-terminal cleavage generates an isoform indistinguishable from the full-length isoform (47 kDa) in SDS-PAGE.

FoxP3 can act as a transcriptional repressor (for IL-2) via an interaction with HDAC7. By contrast, it can also act as a transcriptional activator (for GITRL and
CTLA-4) through an interaction with RUNX7. Notably, for both human and mouse, full-length 47 kDa FoxP3 will induce high levels of CTLA-4 on expressing cells. However, when the C-terminally truncated isoform (at Arg417) is considered, IL-10 promoting activity is enhanced in mouse, while suppressive activity is lost in human. Over aa 105-200, human FoxP3 shares 83% and 84% aa sequence identity with rat and mouse FoxP3, respectively.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Entrez Gene IDs

50943 (Human); 20371 (Mouse)

Gene Symbol

FOXP3

UniProt

Q9BZS1

Additional FoxP3 Products

Product Documents for Human/Mouse/Rat FoxP3 PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat FoxP3 PE‑conjugated Antibody

For research use only

Citations for Human/Mouse/Rat FoxP3 PE‑conjugated Antibody

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FAQs for Human/Mouse/Rat FoxP3 PE‑conjugated Antibody

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  • Q: What is the immunogen sequence of this FoxP3 antibody?

    A: A sequence from Gln105 - Lys200.

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