|Detection of CD4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD4 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB554G, open histogram) or isotype control antibody (Catalog # IC013G, filled histogram). View our protocol for Staining Membrane-associated Proteins.|
|CD4 in Mouse Splenocytes. CD4 was detected and stained in immersion fixed mouse splenocytes using Rat Anti-Mouse CD4 Alexa Fluor® 488‑conjugated Monoclonal Antibody (green, Catalog # FAB554G) at 5 μg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.|
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