Detection of CD4 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD4 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB554G, open histogram) or isotype control antibody (Catalog # IC013G, filled histogram). View our protocol for Staining Membrane-associated Proteins.
CD4 in Mouse Splenocytes.
CD4 was detected and stained in immersion fixed mouse splenocytes using Rat Anti-Mouse CD4 Alexa Fluor® 488‑conjugated Monoclonal Antibody (green, Catalog # FAB554G) at 5 μg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.
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