Mouse CD4 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB554G-100
FAB554G-025
Detection of CD4 in Mouse Splenocytes by Flow Cytometry.
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Citations (1)
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Mouse CD4 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.
Source
Monoclonal Rat IgG2B Clone # GK1.5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse CTL clone V4
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below
Immunocytochemistry
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD4 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB554G, open histogram) or isotype control antibody (Catalog # IC013G, filled histogram). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry CD4 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CD4 in Mouse Splenocytes. CD4 was detected and stained in immersion fixed mouse splenocytes using Rat Anti-Mouse CD4 Alexa Fluor® 488‑conjugated Monoclonal Antibody (green, Catalog # FAB554G) at 5 μg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names
CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Mouse CD4 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-12 signaling drives the differentiation and function of a TH1-derived TFH1-like cell population
    Authors: MD Powell, KA Read, BK Sreekumar, DM Jones, KJ Oestreich
    Sci Rep, 2019;9(1):13991.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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