Human CD4 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3791F-100
FAB3791F-025
Detection of CD4 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (5)
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Reviews (1)

Human CD4 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD4 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 11830
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant Human CD4.
Extracellular Domain
Accession # P01730
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD4 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) or (B) Mouse IgG2AFluorescein Isotype Control (Catalog # IC003F) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names
CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Citations for Human CD4 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Phosphonate-Modified Cellulose Nanocrystals Potentiate the Th1 Polarising Capacity of Monocyte-Derived Dendritic Cells via GABA-B Receptor
    Authors: M Beki?, M Vasiljevi?, D Stojanovi?, V Kokol, D Mihajlovi?, D Vu?evi?, P Uskokovi?, M ?oli?, S Tomi?
    International Journal of Nanomedicine, 2022;17(0):3191-3216.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Aerobic exercise in humans mobilizes HSC in an intensity dependent manner
    Authors: Gianni Parise
    J. Appl. Physiol., 2016;0(0):jap.00696.201.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Impaired circulating CD4+ LAP+ regulatory T cells in patients with acute coronary syndrome and its mechanistic study.
    Authors: Zhu Z, Meng K, Zhong Y, Qi L, Mao X, Yu K, Zhang W, Zhu P, Ren Z, Wu B, Ji Q, Wang X, Zeng Q
    PLoS ONE, 2014;9(2):e88775.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Preferential migration of T regulatory cells induced by IL-16.
    Authors: McFadden C, Morgan R, Rahangdale S, Green D, Yamasaki H, Center D, Cruikshank W
    J. Immunol., 2007;179(10):6439-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Potential role for IL-7 in Fas-mediated T cell apoptosis during HIV infection.
    Authors: Fluur C, De Milito A, Fry TJ, Vivar N, Eidsmo L, Atlas A, Federici C, Matarrese P, Logozzi M, Rajnavolgyi E, Mackall CL, Fais S, Chiodi F, Rethi B
    J. Immunol., 2007;178(8):5340-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human CD4 Fluorescein-conjugated Antibody

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Human CD4 Fluorescein-conjugated Antibody
By Anonymous on 07/07/2017
Application: Flow Sample Tested: Peripheral blood cells Species: Human