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Glutathione Peroxidase 3/GPX3 Alexa Fluor® 750-conjugated Antibody

Human/Mouse/Rat
Glutathione Peroxidase 3/GPX3 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF4199S Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4199S-100UG

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Human/Mouse/Rat
Glutathione Peroxidase 3/GPX3 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Glutathione Peroxidase 3/GPX3 in Western blots and detects recombinant human and recombinant mouse Glutathione Peroxidase 3/GPX3 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recom
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Glutathione Peroxidase 3/GPX3
Gly74-Lys226
Accession # P46412
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Glutathione Peroxidase 3/GPX3

Glutathione Peroxidase 3 (GPX3), also known as Plasma Glutathione Peroxidase, is a 226 amino acid member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX3 is secreted into plasma and like GPX1, is an ~100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. GPX3 exhibits highest levels of mRNA expression in liver (human only), kidney, heart and lung tissues. Mouse GPX3 shares 89% and 99% amino acid identity with human and rat GPX3, respectively.

Entrez Gene IDs
2878 (Human); 14778 (Mouse); 64317 (Rat)
Alternate Names
EC 1.11.1; EC 1.11.1.9; Extracellular glutathione peroxidase; glutathione peroxidase 3 (plasma); Glutathione Peroxidase 3; GPX3; GPx-3; GPXP; GPX-P; GSHPx-3; GSHPx-P; Plasma glutathione peroxidase

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