Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Glutathione Peroxidase 3/GPX3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Glutathione Peroxidase 3/GPX3
Gly74-Lys226
Accession # P46412

Specificity

Detects human, mouse, and rat Glutathione Peroxidase 3/GPX3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Biotinylated Antibody

Detection of Human Glutathione Peroxidase 3/GPX3 antibody by Western Blot.

Detection of Human Glutathione Peroxidase 3/GPX3 by Western Blot.

Western blot shows human serum. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 3/GPX3 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Biotinylated Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human serum

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glutathione Peroxidase 3/GPX3

Glutathione Peroxidase 3 (GPX3), also known as Plasma Glutathione Peroxidase, is a 226 amino acid member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX3 is secreted into plasma and like GPX1, is an ~100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. GPX3 exhibits highest levels of mRNA expression in liver (human only), kidney, heart and lung tissues. Mouse GPX3 shares 89% and 99% amino acid identity with human and rat GPX3, respectively.

Alternate Names

GPX-P, GPX3

Entrez Gene IDs

2878 (Human); 14778 (Mouse); 64317 (Rat)

Gene Symbol

GPX3

UniProt

P46412

Additional Glutathione Peroxidase 3/GPX3 Products

Product Documents for Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Biotinylated Antibody

For research use only

Related Research Areas

Cancer Biomarkers

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