Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western, IF/IHC, Simple Plex Automated Immunoassay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Glutathione Peroxidase 3/GPX3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Glutathione Peroxidase 3/GPX3
Gly74-Lys226
Accession # P46412

Specificity

Detects human, mouse, and rat Glutathione Peroxidase 3/GPX3 in Western blots and detects recombinant human and recombinant mouse Glutathione Peroxidase 3/GPX3 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) GPX5 and recombinant mouse (rm) GPX1 is observed, and less than 4% cross-reactivity with rhGPX4, rhGPX6, and rmGPX2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat
Glutathione Peroxidase 3/GPX3 Antibody

Detection of Human/Mouse/Rat Glutathione Peroxidase 3 antibody by Western Blot.

Detection of Human/Mouse/Rat Glutathione Peroxidase 3 by Western Blot.

Western blot shows lysates of human plasma, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 3 at approximately 24kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Glutathione Peroxidase 3/GPX3 antibody in Human Kidney by Immunohistochemistry (IHC-P).

Glutathione Peroxidase 3/GPX3 in Human Kidney.

Glutathione Peroxidase 3/GPX3 was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4199) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Glutathione Peroxidase 3/GPX3 antibody by Simple WesternTM.

Detection of Human Glutathione Peroxidase 3/GPX3 by Simple WesternTM.

Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for Glutathione Peroxidase 3/GPX3 at approximately 29 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4199) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Glutathione Peroxidase 3/GPX3 by Immunohistochemistry

Detection of Glutathione Peroxidase 3/GPX3 by Immunohistochemistry

Bioinformatics analysis showed that GPX3 expression was significantly increased during adipogenic differentiation of hBMSCs. A Heatmap of adipogenic marker genes and GPx family genes. B Volcano plot of adipogenic marker genes and GPx family genes. C The gene expression pattern of GPx family and oxidative stress-related enzymes during osteogenic and adipogenic differentiation of hBMSCs. Oxidative stress-related enzymes genes: PLA2G2A, LOX, CAT; Adipogenic marker genes: PPARG, CEBPA, FABP4; osteogenic marker genes: ALPL, BGLAP Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40410842), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Glutathione Peroxidase 3/GPX3 by Western Blot

Detection of Glutathione Peroxidase 3/GPX3 by Western Blot

GPx3 expression during adipogenic differentiation of mBMSC in vitro. A Representative images of Oil Red O staining at different differentiation time points and (B) corresponding quantification. C Representative Western blot images showing the protein expressions of GPx3, along with adipogenic markers PPAR gamma and C/EBP alpha, at various differentiation stages. D-F Quantifications of band intensities in (C). G-J Relative mRNA expression levels of Gpx3 and adipogenic-specific genes Pparg, Cebp alpha, and Adipoq normalized to Actb during adipogenic differentiation. Data are presented as mean ± SD. *P < 0.05, ***P < 0.001, ****P < 0.0001 compared to the control group; ns indicates P > 0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40410842), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Glutathione Peroxidase 3/GPX3 by Flow Cytometry

Detection of Glutathione Peroxidase 3/GPX3 by Flow Cytometry

scRNA-seq data indicates that increased BMAds is associated with diminished Gpx3 expression during ageing. A The proportion of bone marrow cells in the young and aged bone marrow. B The expression levels of Adipoq or Lpl in the young and aged bone marrow. C-E UMAP visualization of Adipoq (adipocyte marker), Aspn (BMSC marker) and Gpx3 expression in scRNA-seq data from bone marrow cells of mice at different ages. F The expression levels of Gpx3 in BMSCs and adipocytes in the young and aged mice. 3 M, three-month-old mice; 16 M, sixteen-month-old mice; EC, endothelial cell Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40410842), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/RatGlutathione Peroxidase 3/GPX3 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Simple Western

25 µg/mL
Sample: Human serum

Western Blot

0.5 µg/mL
Sample: Human plasma, mouse kidney tissue, and rat kidney tissue

Reviewed Applications

Read 1 review rated 5 using AF4199 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glutathione Peroxidase 3/GPX3

Glutathione Peroxidase 3 (GPX3), also known as Plasma Glutathione Peroxidase, is a 226 amino acid member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX3 is secreted into plasma and like GPX1, is an ~100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. GPX3 exhibits highest levels of mRNA expression in liver (human only), kidney, heart and lung tissues. Mouse GPX3 shares 89% and 99% amino acid identity with human and rat GPX3, respectively.

Alternate Names

GPX-P, GPX3

Entrez Gene IDs

2878 (Human); 14778 (Mouse); 64317 (Rat)

Gene Symbol

GPX3

UniProt

P46412

Additional Glutathione Peroxidase 3/GPX3 Products

Product Documents for Human/Mouse/RatGlutathione Peroxidase 3/GPX3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/RatGlutathione Peroxidase 3/GPX3 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human/Mouse/Rat
Glutathione Peroxidase 3/GPX3 Antibody

Customer Reviews for Human/Mouse/RatGlutathione Peroxidase 3/GPX3 Antibody (1)

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  • Human/Mouse/Rat
    Glutathione Peroxidase 3/GPX3 Antibody
    Name: Thorsten Cramer
    Application: Immunohistochemistry
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 04/21/2017
    Heat-induced antigen retrieval with citrate buffer antibody concentration 1:400
    Human/Mouse/RatGlutathione Peroxidase 3/GPX3 Antibody AF4199

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