Human/Mouse/Rat HSP70/HSPA1A Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Mouse HSP70/ HSPA1A by Western Blot.
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Product Details
Citations (7)
Supplemental Products
Reviews (1)

Human/Mouse/Rat HSP70/HSPA1A Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects the induced form of human and mouse HSP70/HSPA1A in Western blots. In Western blots, no cross-reactivity with the constitutively expressed HSC70 (HSP73) is detected.
Monoclonal Mouse IgG2A Clone # 242707
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human HSP70/HSPA1A
Accession # NP_005336
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
0.1 µg/mL
See below
Simple Western
0.5 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse HSP70/ HSPA1A antibody by Western Blot. View Larger

Detection of Human and Mouse HSP70/ HSPA1A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with a 42 °C heat shock for 30 minutes with a 3 hour recovery. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP70/HSPA1A at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry HSP70/HSPA1A antibody in Human Liver Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

HSP70/HSPA1A in Human Liver Cancer Tissue. HSP70/HSPA1A was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human HSP70/HSPA1A antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human HSP70/HSPA1A by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) by heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 67 kDa (as indicated) using 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP70/HSPA1A

The heat shock proteins are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Some HSPs are expressed at low levels under normal physiological conditions but show dramatically increased expression in response to cellular stress, others are constitutively expressed. Specific HSPs play a role in regulating apoptosis by interacting directly with key components of the apoptotic pathway.

Long Name
Heat Shock Protein 70
Entrez Gene IDs
3303 (Human); 15511 (Mouse); 24472 (Rat)
Alternate Names
dnaK-type molecular chaperone HSP70-1; FLJ54303; FLJ54370; FLJ54392; FLJ54408; FLJ75127; Heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A/1B; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HSP70; HSP70.1/HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B

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Citations for Human/Mouse/Rat HSP70/HSPA1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. MSC Pretreatment for Improved Transplantation Viability Results in Improved Ventricular Function in Infarcted Hearts
    Authors: MF Pittenger, S Eghtesad, PG Sanchez, X Liu, Z Wu, L Chen, BP Griffith
    International Journal of Molecular Sciences, 2022;23(2):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Heat Shock Proteins HSPA1 and HSP90AA1 Are Upregulated in Colorectal Polyps and Can Be Targeted in Cancer Cells by Anti-Inflammatory Oxicams with Arylpiperazine Pharmacophore and Benzoyl Moiety Substitutions at Thiazine Ring
    Authors: I Szczuka, J Wierzbicki, P Serek, BM Szcz??niak, M Krzystek-K
    Biomolecules, 2021;11(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. Cyclin E1 in Murine and Human Liver Cancer: A Promising Target for Therapeutic Intervention during Tumour Progression
    Authors: R Sonntag, C Penners, M Kohlhepp, U Haas, D Lambertz, A Kroh, T Cramer, F Ticconi, IG Costa, F Tacke, N Gassler, C Trautwein, C Liedtke
    Cancers, 2021;13(22):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers
    Authors: K Dissanayak, M Nõmm, F Lättekivi, Y Ressaissi, K Godakumara, A Lavrits, G Midekessa, J Viil, R Bæk, MM Jørgensen, S Bhattachar, A Andronowsk, A Salumets, Ü Jaakma, A Fazeli
    Theriogenology, 2020;149(0):104-116.
    Species: Mouse
    Sample Types:
    Applications: Microarray
  5. Hsc70/Stub1 promotes the removal of individual oxidatively stressed peroxisomes
    Authors: BH Chen, YJ Chang, S Lin, WY Yang
    Nat Commun, 2020;11(1):5267.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori
    Authors: Guangxin Li
    Am J Transl Res, 2016;8(10):4184-4194.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Western Blot
  7. Exosomes released from macrophages infected with intracellular pathogens stimulate a proinflammatory response in vitro and in vivo.
    Authors: Bhatnagar S, Shinagawa K, Castellino FJ, Schorey JS
    Blood, 2007;110(9):3234-44.
    Species: Human
    Sample Types: Exosomes
    Applications: Western Blot


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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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Human/Mouse/Rat HSP70/HSPA1A Antibody
By Anonymous on 08/19/2021
Application: IHC Sample Tested: Spinal cord Species: Mouse