Human/Mouse/Rat JAB1 Antibody

Catalog # Availability Size / Price Qty
AF6390
AF6390-SP
Human/Mouse/Rat JAB1 Antibody in Western Blot
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Human/Mouse/Rat JAB1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat JAB1 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human JAB1
aa 158-334
Accession # Q92905
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human, Mouse, and Rat JAB1 by Western Blot. View Larger

Detection of Human, Mouse, and Rat JAB1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat JAB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6390) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for JAB1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAB1

JAB1 (Jun activation domain binding protein-1), also called COPS5 or CSN5 (COP9 signalosome-5), is a 334 amino acid (aa), 38 kDa protein that is a subunit of the COP9 signalosome complex. It mediates cytosolic translocation of the cell cycle inhibitory protein, CDKN1B/p27Kip1, for degradation, thus promoting proliferation. It is widely expressed, especially in proliferating cells and up-regulates granulopoiesis when overexpressed. Within the region used as an immunogen, human JAB1 shares 99% aa identity with mouse and rat JAB1. A 379 aa isoform contains 93 alternate aa in place of the N-terminal 48 aa of the 334 aa form.

Long Name
Jun Activation Domain-binding Protein 1
Entrez Gene IDs
10987 (Human); 26754 (Mouse); 312916 (Rat)
Alternate Names
COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis); COP9 signalosome complex subunit 5; COPS5; CSN5; CSN5COP9 (constitutive photomorphogenic, Arabidopsis, homolog) subunit 5; EC 3.4; JAB1; JAB138 kDa Mov34 homolog; Jun activation domain-binding protein 1; MOV-34; SGN5; SGN5MGC3149; Signalosome subunit 5

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