Human/Mouse/Rat mGluR5/1a Antibody

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Detection of mGluR5/1a by Western Blot
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Product Details
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Human/Mouse/Rat mGluR5/1a Antibody Summary

Species Reactivity
Human, Mouse, Rat
Rat ~125 kDa mGluR5 and mGluR1a monomers and ~250 kDa homodimers
Polyclonal Rabbit IgG
Antigen Affinity-purified
Peptide from the C-terminus region of rat mGluR5 and mGluR1a
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.


Recommended Concentration
Western Blot
1:1000 dilution
See below
1:500 dilution
(frozen sections; unpublished observations)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of mGluR5/1a antibody by Western Blot View Larger

Detection of mGluR5/1a by Western Blot Western blot of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125 kDa monomers and the ~250 kDa dimers of mGluR1a and mGluR5.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: mGluR5/1a

Rat metabotropic glutamate receptor 5 (mGluR5b; also mGlu5b) is a 150 kDa, 1183 amino acid (aa), 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. Group I receptors include mGluR1 and mGluR5. Both are postsynaptic, associated with Gq-like proteins, mobilize Ca++ from intracellular stores, and regulate neuronal excitability by influencing ion channel activity. Their activities are not necessarily redundant. Although both mobilize intracellular Ca++ stores, mGluR1 also induces Ca++ influx via TRP channels. On T cells, mGluR1 is inducible and promotes cell proliferation, while mGluR5 is constitutive and blocks cell proliferation. mGluR5 has a large glycosylated extracellular domain (ECD) of 558 aa. The ECD either covalently homodimerizes or heterodimerizes with the Ca++-sensor receptor (CaSR). Dimerization creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. There is one alternate splice form of mGluR5b. It shows a 32 aa deletion between aa 876 - 907 in the cytoplasmic region. The rat mGluR5 C-terminal cytoplasmic region is 90% and 97% aa identical to the equivalent regions in human and mouse mGluR5, respectively.

  1. Pin, J-P. and F. Acher (2002) Curr. Drug Targets CNS Neurol. Disord. 1:297.
  2. Boldyrev, A.A. et al. (2005) J. Neurochem. 95:913.
  3. Ferraguti, F. and R. Shigemoto (2006) Cell Tissue Res. 326:483.
  4. Gama, L. et al. (2001) J. Biol. Chem. 276:39053.
  5. Topolnik, L. et al. (2006) J. Physiol. 575:115.
Long Name
Metabotropic Glutamate Receptor 5 and 1
Alternate Names

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