Human/Mouse/Rat mGluR5/1a Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Rat ~125 kDa mGluR5 and mGluR1a monomers and ~250 kDa homodimers
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Peptide from the C-terminus region of rat mGluR5 and mGluR1a
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
  • Immunohistochemistry
    1:500 dilution
    (frozen sections; unpublished observations)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of mGluR5/1a by Western Blot Western blot of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125 kDa monomers and the ~250 kDa dimers of mGluR1a and mGluR5.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: mGluR5/1a
Rat metabotropic glutamate receptor 5 (mGluR5b; also mGlu5b) is a 150 kDa, 1183 amino acid (aa), 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. Group I receptors include mGluR1 and mGluR5. Both are postsynaptic, associated with Gq-like proteins, mobilize Ca++ from intracellular stores, and regulate neuronal excitability by influencing ion channel activity. Their activities are not necessarily redundant. Although both mobilize intracellular Ca++ stores, mGluR1 also induces Ca++ influx via TRP channels. On T cells, mGluR1 is inducible and promotes cell proliferation, while mGluR5 is constitutive and blocks cell proliferation. mGluR5 has a large glycosylated extracellular domain (ECD) of 558 aa. The ECD either covalently homodimerizes or heterodimerizes with the Ca++-sensor receptor (CaSR). Dimerization creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. There is one alternate splice form of mGluR5b. It shows a 32 aa deletion between aa 876 - 907 in the cytoplasmic region. The rat mGluR5 C-terminal cytoplasmic region is 90% and 97% aa identical to the equivalent regions in human and mouse mGluR5, respectively.
  • References:
    1. Pin, J-P. and F. Acher (2002) Curr. Drug Targets CNS Neurol. Disord. 1:297.
    2. Boldyrev, A.A. et al. (2005) J. Neurochem. 95:913.
    3. Ferraguti, F. and R. Shigemoto (2006) Cell Tissue Res. 326:483.
    4. Gama, L. et al. (2001) J. Biol. Chem. 276:39053.
    5. Topolnik, L. et al. (2006) J. Physiol. 575:115.
  • Long Name:
    Metabotropic Glutamate Receptor 5 and 1
  • Alternate Names:
    mGluR5/1a
Related Research Areas
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit

CTS005 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Anti-Rabbit HRP-AEC Cell & Tissue Staining Kit

CTS006 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 42  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG Antibody

WB AF008 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse/Rat mGluR5/1a Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information