Detection of mGluR5/1a by Western Blot Western blot of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125 kDa monomers and the ~250 kDa dimers of mGluR1a and mGluR5.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Rat metabotropic glutamate receptor 5 (mGluR5b; also mGlu5b) is a 150 kDa, 1183 amino acid (aa), 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. Group I receptors include mGluR1 and mGluR5. Both are postsynaptic, associated with Gq-like proteins, mobilize Ca++ from intracellular stores, and regulate neuronal excitability by influencing ion channel activity. Their activities are not necessarily redundant. Although both mobilize intracellular Ca++ stores, mGluR1 also induces Ca++ influx via TRP channels. On T cells, mGluR1 is inducible and promotes cell proliferation, while mGluR5 is constitutive and blocks cell proliferation. mGluR5 has a large glycosylated extracellular domain (ECD) of 558 aa. The ECD either covalently homodimerizes or heterodimerizes with the Ca++-sensor receptor (CaSR). Dimerization creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. There is one alternate splice form of mGluR5b. It shows a 32 aa deletion between aa 876 - 907 in the cytoplasmic region. The rat mGluR5 C-terminal cytoplasmic region is 90% and 97% aa identical to the equivalent regions in human and mouse mGluR5, respectively.
Pin, J-P. and F. Acher (2002) Curr. Drug Targets CNS Neurol. Disord. 1:297.
Boldyrev, A.A. et al. (2005) J. Neurochem. 95:913.
Ferraguti, F. and R. Shigemoto (2006) Cell Tissue Res. 326:483.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.