Human/Mouse/Rat Mind Bomb 1/MIB1 Antibody

AF7305 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7305
AF7305-SP

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Human/Mouse/Rat Mind Bomb 1/MIB1 Antibody in Western Blot

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Mind Bomb 1/MIB1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat Mind Bomb 1/MIB1 in Western blots and detects recombinant human Mind Bomb 1/MIB1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant MIB2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Mind Bomb 1/MIB1
Thr365-Gln461
Accession # Q86YT6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot

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Detection of Human, Mouse, and Rat Mind Bomb 1/MIB1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Mind Bomb 1/MIB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7305) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Mind Bomb 1/MIB1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mind Bomb 1/MIB1

MIB1 (Mind bomb 1; also DIP-1 and ZZANK2) is a 110-115 kDa E3 ubiquitin ligase. It is widely expressed in both embryo and adult, and appears to have a non-redundant role in Notch receptor signaling. Notch signaling is initiated when a transmembrane (TM) Notch ligand (Jagged or Delta) binds to Notch on an adjacent cell. This ligation induces cleavage of the Notch extracellular domain (ECD) with the creation of a Jagged/Delta:NotchECD complex. This complex is now internalized by the Notch ligand-expressing cell. The displacement of the NotchECD from its TM segment promotes further Notch processing, resulting in Notch signaling. The key internalization step by the ligand-expressing cell is mediated by the MIB1 E3 ubiquitin ligase. Human MIB1 is 1006 amino acids (aa) in length. It contains multiple interspersed Ankyrin repeats, MIB domains, and zinc-finger motifs. There is one potential alternative start site at Met84. Over aa 365-461, human MIB1 shares 99% aa sequence identity with mouse MIB1.

Long Name

Mindbomb Homolog 1

Entrez Gene IDs

57534 (Human); 225164 (Mouse); 307594 (Rat)

Alternate Names

DAPK-interacting protein 1; DIP1; DIP-1FLJ90676; DKFZp686I0769; DKFZp761M1710; E3 ubiquitin-protein ligase MIB1; EC 6.3.2; EC 6.3.2.-; KIAA1323; MGC129660; MIB; MIB1; Mind Bomb 1; Mind bomb homolog 1; mindbomb homolog 1 (Drosophila); ubiquitin ligase mind bomb; ubiquitin ligase protein MIB1; Zinc finger ZZ type with ankyrin repeat domain protein 2; ZZANK2; ZZANK2MGC129659; ZZZ6