Human/Mouse/Rat Netrin-1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat Netrin‑1 by Western Blot.
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Product Details
Citations (5)
Supplemental Products

Human/Mouse/Rat Netrin-1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat Netrin-1 in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Netrin-1
Accession # O95631
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Netrin-1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Netrin‑1 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Netrin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6419) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Netrin-1 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-1

Netrin-1 (from Sanskrit netr meaning "to guide"; also known as Epididymis tissue protein Li 131P) is a 66-76 kDa secreted member of the laminin superfamily of molecules. It is expressed by multiple cell types, including embryonic neural tube ventral midline cells plus postnatal renal tubule epithelium and oligodendroglia. Netrin-1 has multiple receptors, including beta 4-integrins, DCC, DSCAM and UNC5A-thru-D. Its effects are context specific. For example, on neurons, binding to either DCC or DSCAM alone promotes chemoattraction, while a DCC:UNC5 complex promotes chemorepulsion. In addition, the ligation of UNC5B on leukocytes suppresses cytokine production, while the binding of netrin to DCC expressed on select cell types inhibits apoptosis. Mature human netrin-1 is 580 amino acids (aa) in length (aa 25-604). It contains an N-terminal laminin domain (aa 47-284), three EGF-like domains (aa 285-453) and a C-terminal cell-interaction netrin-like domain (aa 472-601). Over aa 22-604, human netrin-1 shares 99% aa sequence identity with mouse netrin-1.

Entrez Gene IDs
9423 (Human); 18208 (Mouse); 396389 (Chicken)
Alternate Names
netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of

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Citations for Human/Mouse/Rat Netrin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Netrin-1 Stimulates Migration of Neogenin Expressing Aggressive Melanoma Cells
    Authors: G Untiveros, A Raskind, L Linares, A Dotti, L Strizzi
    International Journal of Molecular Sciences, 2022;23(21):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth
    Authors: B Mondal, H Jin, S Kallappago, Y Sedkov, T Martinez, MF Sentmanat, GJ Poet, C Li, Y Fan, SM Pruett-Mil, HM Herz
    Elife, 2020;9(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus
    Authors: CP Prieto, BS Casas, P Falcón, A Villanueva, P Lois, J Lattus, V Palma
    Int J Mol Sci, 2019;20(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton's jelly mesenchymal stem cells (WJ-MSC)
    Authors: CP Prieto, MC Ortiz, A Villanueva, C Villarroel, SS Edwards, M Elliott, J Lattus, S Aedo, D Meza, P Lois, V Palma
    Stem Cell Res Ther, 2017;8(1):43.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. The Netrin-4/ Neogenin-1 axis promotes neuroblastoma cell survival and migration
    Authors: AA Villanueva, P Falcón, N Espinoza, LS R, LA Milla, E Hernandez-, VA Torres, P Sanchez-Go, V Palma
    Oncotarget, 2017;8(6):9767-9782.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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