Human/Mouse/Rat Neural 3-Color Immunocytochemistry Kit

Why Analyze Neural Progenitor Cell Proliferation and Differentiation In Vitro?

Research studies using neural progenitor cells (NPCs) most often require weeks of cell culture before an experimental hypothesis can be tested.

Thus, the ability to verify multipotency at an early stage in the experimental process is an important time saving step. Multipotency status can be evaluated by the presence of cell markers and/or the ability of progenitor cells to differentiate into multiple neural lineages. To determine if a cell population truly consists of multipotent NPCs, it is important to verify their ability to differentiate into neurons, astrocytes, and oligodendrocytes.

3-color ICC using neural cell markers:

• Evaluates the multipotency status of the starting NPC population.
• Increases confidence in multipotency status by combining 3 different fluorochrome-conjugated antibodies for simultaneous staining.
• Reduces variability within an experiment and between studies.
• Ensures efficient use of time and reagents by utilizing single-step staining.
• Can be performed using randomly differentiated samples.

This kit contains the following fluorochrome-conjugated antibodies to verify neural stem cell multipotency:

• NL557-conjugated Mouse anti-Human Oligodendrocyte Marker O4
• NL637-conjugated Mouse anti-beta III Tubulin
• NL493-conjugated Sheep anti-Human GFAP

Each antibody is supplied as a 10X stock; enough for 25 assays when used in a 50 µL staining volume per assay.