Human/Mouse/Rat NF-L Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB2216S Datasheet / COA / SDS

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FAB2216S-100UG

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Human/Mouse/Rat NF-L Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human NF-L in direct ELISAs and human, mouse and rat NF-L in Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) NF‑H or rhNF‑M is observed.

Source

Monoclonal Mouse IgG2b Clone # 268014

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human NF-L
Met1-Asp338
Accession # P07196

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NF-L

NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that is expressed in neurons. It associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

Long Name

Neurofilament Protein, Light Chain

Entrez Gene IDs

4747 (Human)

Alternate Names

68 kDa neurofilament protein; CMT2E; NEFL; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament-light; NF68; NF68FLJ53642; NFL; NF-L; NFLlight polypeptide 68kDa