Human/Mouse/Rat p38 beta Antibody

Catalog #: MAB5885 2 Reviews Datasheet / COA / SDS

Discontinued Product

MAB5885 has been discontinued.

View all p38 beta/MAPK11 products.

Detection of Human, Mouse, and Rat p38 beta by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat p38 beta Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat p38 beta in Western blots.

Source

Monoclonal Mouse IgG₃ Clone # 538307

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human p38 beta
Met1-Thr107
Accession # NP_002742

Formulation

Lyophilized from a 0.2 μm filtered solution in Borate and NaCl with Trehalose.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat p38 beta by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat p38 beta Monoclonal Antibody (Catalog # MAB5885) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p38 beta at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Western Blot

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Detection of Human p38 beta by Western Blot. Western blot shows Recombinant Human p38 beta, Recombinant Human p38?, Recombinant Human p38d, and Recombinant Active Human p38a (Catalog # 5477-KS) (10 ng/lane). PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat p38 beta Monoclonal Antibody (Catalog # MAB5885) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38 beta/MAPK11

The p38 mitogen-activated protein kinases (p38 MAP kinases) are a family of four related Ser/Thr kinases responsive to pro-inflammatory cytokines and environmental stresses, including ionizing radiation, oxidative stress, and osmotic shock. Each family member, p38 alpha, p38 beta, p38δ, and p38 gamma, is activated by dual Thr and Tyr phosphorylation within a Thr-Gly-Tyr motif residing in the kinase activation loop. For p38 beta, also known as p38-2, stress-activated protein kinase 2B (SAPK2B), and MAPK11, this dual phosphorylation occurs at Thr180/Tyr182.

Long Name

Mitogen-Activated Protein Kinase 11

Entrez Gene IDs

5600 (Human); 19094 (Mouse); 689314 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.24; MAP kinase 11; MAP kinase p38 beta; MAPK 11; MAPK11; mitogen-activated protein kinase 11; Mitogen-activated protein kinase p38 beta; mitogen-activated protein kinase p38-2; MPK11; p38 beta; p38b; p38beta; P38BETA2; PRKM11; SAPK2B; SAPK2p38-2P38B; Stress-activated protein kinase 2; stress-activated protein kinase-2; stress-activated protein kinase-2b