Human/Mouse/Rat p38 beta Antibody
Human/Mouse/Rat p38 beta Antibody Summary
Accession # NP_002742
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat p38 beta by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat p38 beta Monoclonal Antibody (Catalog # MAB5885) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p38 beta at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Human p38 beta by Western Blot. Western blot shows Recombinant Human p38 beta, Recombinant Human p38?, Recombinant Human p38d, and Recombinant Active Human p38a (Catalog # 5477-KS) (10 ng/lane). PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat p38 beta Monoclonal Antibody (Catalog # MAB5885) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p38 beta/MAPK11
The p38 mitogen-activated protein kinases (p38 MAP kinases) are a family of four related Ser/Thr kinases responsive to pro-inflammatory cytokines and environmental stresses, including ionizing radiation, oxidative stress, and osmotic shock. Each family member, p38 alpha, p38 beta, p38δ, and p38 gamma, is activated by dual Thr and Tyr phosphorylation within a Thr-Gly-Tyr motif residing in the kinase activation loop. For p38 beta, also known as p38-2, stress-activated protein kinase 2B (SAPK2B), and MAPK11, this dual phosphorylation occurs at Thr180/Tyr182.
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