Human/Mouse/Rat PALS1/MPP5 Antibody AF7979: R&D Systems

Human/Mouse/Rat PALS1/MPP5 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat PALS1/MPP5 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PALS1/MPP5
    Ala392-Ser509
    Accession # Q8N3R9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat PALS1/MPP5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, bEnd.3 mouse endothelioma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat PALS1/MPP5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7979) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PALS1/MPP5 at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PALS1/MPP5

MPP5 (Membrane Palmitoylated Protein 5; also PALS1/Proteins Associated with Lin7 #1) is a 70-80 kDa member of MAGUK (Membrane-Associated Guanylate Kinase) family of proteins. It is ubiquitously expressed, and plays a key role in the creation of cell polarity and adhesion. Although not its only function, MPP5 is essential for tight junction integrity. Crumbs proteins (Crb-1 and -3) are apically-oriented, integral membrane proteins. These transmembrane proteins are known to bind to cytosolic MPP5, which, in turn, links to PATJ, which, in turn, binds to either cytosolic ZO-3, or to 4-transmembrane laterally-embedded claudin-1. Subsequent binding of ZO-3 with actin unite the cytoskeleton with the cell membrane. Human MPP5 is 675 amino acids (aa) in length. It contains a utilized phosphorylation site at Ser25, two consecutive L27 domains (aa 120-177 and 179-235) that bind PATJ, one PDZ domain (aa 256-336) that binds crumb proteins, an SH3 region (aa 345-417), and a C‑terminal guanylate kinase-like domain (aa 479-660). MPP5 forms both homodimers and heterodimers with MPP4. There is one splice variant that utilizes an alternative start site at Met35. Over aa 392-509, human and mouse MPP5 are identical in amino acid sequence. Overall, human and mouse MPP5 share 97% aa sequence identity.

  • Long Name:
    Protein Associated with Lin Seven 1
  • Entrez Gene IDs:
    64398 (Human); 56217 (Mouse); 314259 (Rat)
  • Alternate Names:
    MAGUK p55 subfamily member 5; membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5); MPP5; PALS1; PALS1FLJ12615; Stardust
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse/Rat PALS1/MPP5 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information