Human/Mouse/Rat PNUTS Antibody Summary
Accession # NP_002705
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse and Rat PNUTS by Western Blot. Western blot shows lysates of TS1 mouse helper T cell line and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat PNUTS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF21581) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PNUTS at approximately 114 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Detection of Human PNUTS by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for PNUTS at approximately 100-150 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/Rat PNUTS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF21581). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
PP1 nuclear targeting subunit (PNUTS), also known as PPP1R10 and FB19, is a 99 kDa protein that binds the serine/threonine protein phosphatase PP1 and causes its localization to chromatin in the nucleus. C-terminal RGG repeats in PNUTS suggest it may bind to single stranded RNA. The PP1/PNUTS complex may also play a role in regulating the cell cycle-dependent phosphorylation of the oncogene Rb.
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