Normal Rabbit IgG Control

Catalog # Availability Size / Price Qty
AB-105-C
Detection of Rabbit IgGControl by Flow Cytometry
1 Image
Product Details
Citations (46)
FAQs
Supplemental Products
Reviews (9)

Normal Rabbit IgG Control Summary

Specificity
Serum was obtained from naive (non-immunized) rabbits and purified for use as normal rabbit IgG.
Source
Polyclonal Rabbit IgG
Purification
Protein A or G purified
Immunogen
NA
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Rabbit IgGControl by Flow Cytometry HepG2 humanhepatocellular carcinoma cell line was stained with Rabbit Anti-HumanKlotho beta Monoclonal Antibody (Catalog # MAB58891,filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # AB-105-C,open histogram), followed byAllophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #F0111). View our protocol for StainingMembrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Dissolve the lyophilized rabbit IgG in sterile PBS, pH 7.4. If 1 mL of PBS is used, the antibody concentration will be 1 mg/mL.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Normal Rabbit IgG Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

46 Citations: Showing 1 - 10
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  1. MiR-497?195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch and HIF-1? activity
    Authors: M Yang, CJ Li, X Sun, Q Guo, Y Xiao, T Su, ML Tu, H Peng, Q Lu, Q Liu, HB He, TJ Jiang, MX Lei, M Wan, X Cao, XH Luo
    Nat Commun, 2017;8(0):16003.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Interactions between fibroblastic reticular cells and B cells promote mesenteric lymph node lymphangiogenesis
    Authors: L Kumar Dube, P Karempudi, SA Luther, B Ludewig, NL Harris
    Nat Commun, 2017;8(1):367.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  3. Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complex
    Authors: SS Ganaie, W Zou, P Xu, X Deng, S Kleiboeker, J Qiu
    PLoS Pathog., 2017;13(5):e1006370.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  4. Cyclodextrin Reduces Cholesterol Crystal-Induced Inflammation by Modulating Complement Activation
    Authors: SS Bakke, MH Aune, N Niyonzima, K Pilely, L Ryan, M Skjelland, P Garred, P Aukrust, B Halvorsen, E Latz, JK Damås, TE Mollnes, T Espevik
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: Flow
  5. MicroRNA-30b controls endothelial cell capillary morphogenesis through regulation of transforming growth factor beta 2
    Authors: GA Howe, K Kazda, CL Addison
    PLoS ONE, 2017;12(10):e0185619.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  6. Tumor-infiltrating monocytes/macrophages promote tumor invasion and migration by upregulating S100A8 and S100A9 expression in cancer cells
    Authors: SY Lim, AE Yuzhalin, AN Gordon-Wee, RJ Muschel
    Oncogene, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Frozen
  7. Neutrophils suppress intraluminal NK-mediated tumor cell clearance and enhance extravasation of disseminated carcinoma cells
    Authors: A Spiegel, MW Brooks, S Houshyar, F Reinhardt, M Ardolino, E Fessler, MB Chen, JA Krall, J DeCock, IK Zervantona, A Iannello, Y Iwamoto, V Cortez-Ret, RD Kamm, MJ Pittet, DH Raulet, RA Weinberg
    Cancer Discov, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. The mitochondrial protein CHCHD2 primes the differentiation potential of human induced pluripotent stem cells to neuroectodermal lineages
    Authors: L Zhu, A Gomez-Dura, G Saretzki, S Jin, K Tilgner, D Melguizo-S, G Anyfantis, J Al-Aama, L Vallier, P Chinnery, M Lako, L Armstrong
    J. Cell Biol., 2016;215(2):187-202.
    Species: Human
    Sample Types: Protein
    Applications: ChIP
  9. Dux4 controls migration of mesenchymal stem cells through the Cxcr4-Sdf1 axis
    Authors: P Dmitriev, E Kiseleva, O Kharchenko, E Ivashkin, A Pichugin, P Dessen, T Robert, F Coppée, A Belayew, G Carnac, D Laoudj-Che, M Lipinski, A Vasiliev, YS Vassetzky
    Oncotarget, 2016;7(40):65090-65108.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ctrl
  10. TGF-? Neutralization Enhances AngII-Induced Aortic Rupture and Aneurysm in Both Thoracic and Abdominal Regions
    Authors: X Chen, DL Rateri, DA Howatt, A Balakrishn, JJ Moorleghen, LA Cassis, A Daugherty
    PLoS ONE, 2016;11(4):e0153811.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  11. Discovery of molecular markers to discriminate corneal endothelial cells in the human body.
    Authors: Yoshihara M, Ohmiya H, Hara S, Kawasaki S, Hayashizaki Y, Itoh M, Kawaji H, Tsujikawa M, Nishida K
    PLoS ONE, 2015;10(3):e0117581.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  12. A discrete chromatin loop in the mouse Tcra-Tcrd locus shapes the TCRdelta and TCRalpha repertoires.
    Authors: Chen L, Carico Z, Shih H, Krangel M
    Nat Immunol, 2015;16(10):1085-93.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  13. Specification of Vdelta and Valpha usage by Tcra/Tcrd locus V gene segment promoters.
    Authors: Naik A, Hawwari A, Krangel M
    J Immunol, 2015;194(2):790-4.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP control
  14. Bmi1 limits dilated cardiomyopathy and heart failure by inhibiting cardiac senescence.
    Authors: Gonzalez-Valdes I, Hidalgo I, Bujarrabal A, Lara-Pezzi E, Padron-Barthe L, Garcia-Pavia P, Gomez-del Arco P, Redondo J, Ruiz-Cabello J, Jimenez-Borreguero L, Enriquez J, de la Pompa J, Hidalgo A, Gonzalez S
    Nat Commun, 2015;6(0):6473.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  15. MicroRNA-188 regulates age-related switch between osteoblast and adipocyte differentiation.
    Authors: Li, Chang-Ju, Cheng, Peng, Liang, Meng-Ke, Chen, Yu-Si, Lu, Qiong, Wang, Jin-Yu, Xia, Zhu-Ying, Zhou, Hou-De, Cao, Xu, Xie, Hui, Liao, Er-Yuan, Luo, Xiang-Ha
    J Clin Invest, 2015;125(4):1509-22.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  16. HEB associates with PRC2 and SMAD2/3 to regulate developmental fates.
    Authors: Yoon, Se-Jin, Foley, Joseph W, Baker, Julie C
    Nat Commun, 2015;6(0):6546.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Co-immunoprecipitation
  17. Th17-type cytokines, IL-6 and TNF-alpha synergistically activate STAT3 and NF-kB to promote colorectal cancer cell growth.
    Authors: De Simone V, Franze E, Ronchetti G, Colantoni A, Fantini M, Di Fusco D, Sica G, Sileri P, MacDonald T, Pallone F, Monteleone G, Stolfi C
    Oncogene, 2015;34(27):3493-503.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  18. Convergence of cMyc and beta-catenin on Tcf7l1 enables endoderm specification.
    Authors: Morrison G, Scognamiglio R, Trumpp A, Smith A
    EMBO J, 2015;35(3):356-68.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  19. CD4(+)Foxp3(+) Tregs protect against innate immune cell-mediated fulminant hepatitis in mice.
    Authors: Hou X, Song J, Su J, Huang D, Gao W, Yan J, Shen J
    Mol Immunol, 2015;63(2):420-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neut Ctrl
  20. Optimisations and Challenges Involved in the Creation of Various Bioluminescent and Fluorescent Influenza A Virus Strains for In Vitro and In Vivo Applications.
    Authors: Spronken M, Short K, Herfst S, Bestebroer T, Vaes V, van der Hoeven B, Koster A, Kremers G, Scott D, Gultyaev A, Sorell E, de Graaf M, Barcena M, Rimmelzwaan G, Fouchier R
    PLoS ONE, 2015;10(8):e0133888.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Ctrl
  21. A transient wave of BMP signaling in the retina is necessary for Muller glial differentiation.
    Authors: Ueki Y, Wilken M, Cox K, Chipman L, Bermingham-McDonogh O, Reh T
    Development, 2015;142(3):533-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ChIP
  22. T-cell receptor alpha enhancer is inactivated in alphabeta T lymphocytes.
    Authors: del Blanco B, Angulo U, Krangel M, Hernandez-Munain C
    Proc Natl Acad Sci U S A, 2015;112(14):E1744-53.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IP
  23. An anti-silencer- and SATB1-dependent chromatin hub regulates Rag1 and Rag2 gene expression during thymocyte development.
    Authors: Hao B, Naik A, Watanabe A, Tanaka H, Chen L, Richards H, Kondo M, Taniuchi I, Kohwi Y, Kohwi-Shigematsu T, Krangel M
    J Exp Med, 2015;212(5):809-24.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IP
  24. Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes.
    Authors: Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier R
    J Neuroinflammation, 2015;12(0):119.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Not Specified
  25. Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts.
    Authors: Angelucci C, Maulucci G, Colabianchi A, Iacopino F, D'Alessio A, Maiorana A, Palmieri V, Papi M, De Spirito M, Di Leone A, Masetti R, Sica G
    Br J Cancer, 2015;112(10):1675-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut Ctrl
  26. Nerve growth factor and proNGF simultaneously promote symmetric self-renewal, quiescence, and epithelial to mesenchymal transition to enlarge the breast cancer stem cell compartment.
    Authors: Tomellini E, Touil Y, Lagadec C, Julien S, Ostyn P, Ziental-Gelus N, Meignan S, Lengrand J, Adriaenssens E, Polakowska R, Le Bourhis X
    Stem Cells, 2015;33(2):342-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC Ctrl
  27. Suppression of Hyperactive Immune Responses Protects against Nitrogen Mustard Injury.
    Authors: Au L, Meisch J, Das L, Binko A, Boxer R, Wen A, Steinmetz N, Lu K
    J Invest Dermatol, 2015;135(12):2971-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  28. Characterization of In Vitro Engineered Human Adipose Tissues: Relevant Adipokine Secretion and Impact of TNF-alpha.
    Authors: Aubin K, Safoine M, Proulx M, Audet-Casgrain M, Côté J, Tetu F, Roy A, Fradette J
    PLoS ONE, 2015;10(9):e0137612.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Ctrl
  29. The CLEC-2-podoplanin axis controls the contractility of fibroblastic reticular cells and lymph node microarchitecture.
    Authors: Astarita, Jillian, Cremasco, Viviana, Fu, Jianxin, Darnell, Max C, Peck, James R, Nieves-Bonilla, Janice M, Song, Kai, Kondo, Yuji, Woodruff, Matthew, Gogineni, Alvin, Onder, Lucas, Ludewig, Burkhard, Weimer, Robby M, Carroll, Michael, Mooney, David J, Xia, Lijun, Turley, Shannon
    Nat Immunol, 2015;16(1):75-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Ctrl
  30. NADPH oxidase-independent formation of extracellular DNA traps by basophils.
    Authors: Morshed M, Hlushchuk R, Simon D, Walls A, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon H, Yousefi S
    J Immunol, 2014;192(11):5314-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype control
  31. Proteomic analysis of the action of the Mycobacterium ulcerans toxin mycolactone: targeting host cells cytoskeleton and collagen.
    Authors: Gama J, Ohlmeier S, Martins T, Fraga A, Sampaio-Marques B, Carvalho M, Proenca F, Silva M, Pedrosa J, Ludovico P
    PLoS Negl Trop Dis, 2014;8(8):e3066.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  32. Cholesterol crystals induce complement-dependent inflammasome activation and cytokine release.
    Authors: Samstad E, Niyonzima N, Nymo S, Aune M, Ryan L, Bakke S, Lappegard K, Brekke O, Lambris J, Damas J, Latz E, Mollnes T, Espevik T
    J Immunol, 2014;192(6):2837-45.
    Species: Human
    Sample Types:
    Applications: Isotype control
  33. Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF.
    Authors: Bernard M, Dieude M, Yang B, Hamelin K, Underwood K, Hebert M
    Autophagy, 2014;10(12):2193-207.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  34. In vivo neuronal action potential recordings via three-dimensional microscale needle-electrode arrays.
    Authors: Fujishiro A, Kaneko H, Kawashima T, Ishida M, Kawano T
    Sci Rep, 2014;4(0):4868.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  35. Developmentally programmed 3' CpG island methylation confers tissue- and cell-type-specific transcriptional activation.
    Authors: Yu, Da-Hai, Ware, Carol, Waterland, Robert A, Zhang, Jiexin, Chen, Miao-Hsu, Gadkari, Manasi, Kunde-Ramamoorthy, Govindar, Nosavanh, Lagina M, Shen, Lanlan
    Mol Cell Biol, 2013;33(9):1845-58.
    Species: Human
    Sample Types: Cell Lysates
    Applications: IP
  36. A soluble fucose-specific lectin from Aspergillus fumigatus conidia--structure, specificity and possible role in fungal pathogenicity.
    Authors: Houser J, Komarek J, Kostlanova N, Cioci G, Varrot A, Kerr S, Lahmann M, Balloy V, Fahy J, Chignard M, Imberty A, Wimmerova M
    PLoS ONE, 2013;8(12):e83077.
    Species: Fungus - Aspergillus fumigatus
    Sample Types: Whole Tissue
    Applications: IHC
  37. CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
    Authors: Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C
    Mucosal Immunol, 2012;5(0):702.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ctrl
  38. Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
    Authors: Yamakawa N, Uchida T, Matthay MA, Makita K
    Am. J. Physiol. Lung Cell Mol. Physiol., 2011;300(4):L516-25.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Ctrl
  39. Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer.
    Authors: Chauchereau A, Al Nakouzi N, Gaudin C, Le Moulec S, Compagno D, Auger N, Benard J, Opolon P, Rozet F, Validire P, Fromont G, Fizazi K
    Exp. Cell Res., 2011;317(3):262-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow control
  40. The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.
    Authors: Suzuki K, Maruya M, Kawamoto S
    Immunity, 2010;33(1):71-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Ctrl
  41. Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose.
    Authors: Harrison ML, Obermueller E, Maisey NR, Hoare S, Edmonds K, Li NF, Chao D, Hall K, Lee C, Timotheadou E, Charles K, Ahern R, King DM, Eisen T, Corringham R, DeWitte M, Balkwill F, Gore M
    J. Clin. Oncol., 2007;25(29):4542-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Ctrl
  42. Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.
    Authors: Bolovan-Fritts CA, Spector SA
    Blood, 2007;111(1):175-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ctrl
  43. Increase in transforming growth factor-beta in the brain during infection is related to fever, not depression of spontaneous motor activity.
    Authors: Matsumura S, Shibakusa T, Fujikawa T, Yamada H, Inoue K, Fushiki T
    Neuroscience, 2007;144(3):1133-40.
    Species: Rat
    Sample Types: CSF
    Applications: Ctrl
  44. Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates.
    Authors: Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ
    J. Immunol., 2004;172(9):5753-64.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Ctrl
  45. Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10.
    Authors: Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA
    J. Immunol., 2004;172(9):5213-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ctrl
  46. Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2.
    Authors: Viti J, Gulacsi A, Lillien L
    J. Neurosci., 2003;23(13):5919-27.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Ctrl

FAQs

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Reviews for Normal Rabbit IgG Control

Average Rating: 4.2 (Based on 9 Reviews)

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Normal Rabbit IgG Control
By Anonymous on 11/05/2018
Application: Flow Sample Tested: SK-BR-3 human breast cancer cell line Species: Human

Normal Rabbit IgG Control
By Anonymous on 04/20/2018
Application: Flow Sample Tested: Human Cancer cell line Species: Human

Normal Rabbit IgG Control
By Anonymous on 08/24/2017
Application: Flow Sample Tested: Adult splenocytes Species: Mouse

Isotype control


Normal Rabbit IgG Control
By Anonymous on 06/17/2017
Application: B/N Sample Tested: Peritoneal macrophages Species: Human

Normal Rabbit IgG Control
By Anonymous on 05/22/2017
Application: ICC/IF Sample Tested: Neutrophils Species: Human

Very disappointed, the antibody is too noisy for IF with human neutrophils to be used as a control, compared to another IgG control that we have.ER

Technical Service is following up


Normal Rabbit IgG Control
By Anonymous on 10/11/2016
Application: ICC/IF Sample Tested: mouse P14 Species: Mouse,Rabbit

used as negative control


Normal Rabbit IgG Control
By Anonymous on 07/27/2016
Application: Functional Assay Sample Tested: mouse lung Species: Mouse

Normal Rabbit IgG Control
By Anonymous on 06/28/2016
Application: Flow Sample Tested: primary mouse cells Species: Mouse

Normal Rabbit IgG Control
By Anonymous on 02/22/2016
Application: Flow Sample Tested: Mouse lymphocytes Species: input species here