Normal Rabbit IgG Control

(23 citations)
(3 Reviews)
  
  • Specificity
    Serum was obtained from naive (non-immunized) rabbits and purified for use as normal rabbit IgG.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Protein A or G purified
  • Immunogen
    NA
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rabbit IgGControl by Flow Cytometry HepG2 humanhepatocellular carcinoma cell line was stained with Rabbit Anti-HumanKlotho beta Monoclonal Antibody (Catalog # MAB58891,filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # AB-105-C,open histogram), followed byAllophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #F0111). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Dissolve the lyophilized rabbit IgG in sterile PBS, pH 7.4. If 1 mL of PBS is used, the antibody concentration will be 1 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 10 of 23
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Species
Applications
Sample Type
  1. Optimisations and Challenges Involved in the Creation of Various Bioluminescent and Fluorescent Influenza A Virus Strains for In Vitro and In Vivo Applications.
    Authors: Spronken M, Short K, Herfst S, Bestebroer T, Vaes V, van der Hoeven B, Koster A, Kremers G, Scott D, Gultyaev A, Sorell E, de Graaf M, Barcena M, Rimmelzwaan G, Fouchier R
    PLoS ONE, 2015;10(8):e0133888.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Ctrl
  2. Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts.
    Authors: Angelucci C, Maulucci G, Colabianchi A, Iacopino F, D'Alessio A, Maiorana A, Palmieri V, Papi M, De Spirito M, Di Leone A, Masetti R, Sica G
    Br J Cancer, 2015;112(10):1675-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut Ctrl
  3. Nerve growth factor and proNGF simultaneously promote symmetric self-renewal, quiescence, and epithelial to mesenchymal transition to enlarge the breast cancer stem cell compartment.
    Authors: Tomellini E, Touil Y, Lagadec C, Julien S, Ostyn P, Ziental-Gelus N, Meignan S, Lengrand J, Adriaenssens E, Polakowska R, Le Bourhis X
    Stem Cells, 2015;33(2):342-53.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Ctrl
  4. Bmi1 limits dilated cardiomyopathy and heart failure by inhibiting cardiac senescence.
    Authors: Gonzalez-Valdes I, Hidalgo I, Bujarrabal A, Lara-Pezzi E, Padron-Barthe L, Garcia-Pavia P, Gomez-del Arco P, Redondo J, Ruiz-Cabello J, Jimenez-Borreguero L, Enriquez J, de la Pompa J, Hidalgo A, Gonzalez S
    Nat Commun, 2015;6(0):6473.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. A discrete chromatin loop in the mouse Tcra-Tcrd locus shapes the TCRdelta and TCRalpha repertoires.
    Authors: Chen L, Carico Z, Shih H, Krangel M
    Nat Immunol, 2015;16(10):1085-93.
    Species: Mouse
    Sample Type: Cell Lysate
    Application: ChIP
  6. Characterization of In Vitro Engineered Human Adipose Tissues: Relevant Adipokine Secretion and Impact of TNF-alpha.
    Authors: Aubin K, Safoine M, Proulx M, Audet-Casgrain M, Côté J, Tetu F, Roy A, Fradette J
    PLoS ONE, 2015;10(9):e0137612.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Ctrl
  7. Suppression of Hyperactive Immune Responses Protects against Nitrogen Mustard Injury.
    Authors: Au L, Meisch J, Das L, Binko A, Boxer R, Wen A, Steinmetz N, Lu K
    J Invest Dermatol, 2015;135(12):2971-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  8. T-cell receptor alpha enhancer is inactivated in alphabeta T lymphocytes.
    Authors: del Blanco B, Angulo U, Krangel M, Hernandez-Munain C
    Proc Natl Acad Sci U S A, 2015;112(14):E1744-53.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IP
  9. Convergence of cMyc and beta-catenin on Tcf7l1 enables endoderm specification.
    Authors: Morrison G, Scognamiglio R, Trumpp A, Smith A
    EMBO J, 2015;35(3):356-68.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ChIP
  10. Specification of Vdelta and Valpha usage by Tcra/Tcrd locus V gene segment promoters.
    Authors: Naik A, Hawwari A, Krangel M
    J Immunol, 2015;194(2):790-4.
    Species: Mouse
    Sample Type: Cell Extracts
    Application: ChIP control
  11. A transient wave of BMP signaling in the retina is necessary for Muller glial differentiation.
    Authors: Ueki Y, Wilken M, Cox K, Chipman L, Bermingham-McDonogh O, Reh T
    Development, 2015;142(3):533-43.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ChIP
  12. The CLEC-2-podoplanin axis controls the contractility of fibroblastic reticular cells and lymph node microarchitecture.
    Authors: Astarita, Jillian, Cremasco, Viviana, Fu, Jianxin, Darnell, Max C, Peck, James R, Nieves-Bonilla, Janice M, Song, Kai, Kondo, Yuji, Woodruff, Matthew, Gogineni, Alvin, Onder, Lucas, Ludewig, Burkhard, Weimer, Robby M, Carroll, Michael, Mooney, David J, Xia, Lijun, Turley, Shannon
    Nat Immunol, 2015;16(1):75-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  13. CD4(+)Foxp3(+) Tregs protect against innate immune cell-mediated fulminant hepatitis in mice.
    Authors: Hou X, Song J, Su J, Huang D, Gao W, Yan J, Shen J
    Mol Immunol, 2015;63(2):420-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut Ctrl
  14. CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
    Authors: Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C
    Mucosal Immunol, 2012;5(0):702.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  15. Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer.
    Authors: Chauchereau A, Al Nakouzi N, Gaudin C, Le Moulec S, Compagno D, Auger N, Benard J, Opolon P, Rozet F, Validire P, Fromont G, Fizazi K
    Exp. Cell Res., 2011;317(3):262-75.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  16. Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
    Authors: Yamakawa N, Uchida T, Matthay MA, Makita K
    Am. J. Physiol. Lung Cell Mol. Physiol., 2011;300(4):L516-25.
    Species: Rat
    Sample Type: Whole Cells
    Application: Ctrl
  17. The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.
    Authors: Suzuki K, Maruya M, Kawamoto S
    Immunity, 2010;33(1):71-83.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  18. Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.
    Authors: Bolovan-Fritts CA, Spector SA
    Blood, 2007;111(1):175-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  19. Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose.
    Authors: Harrison ML, Obermueller E, Maisey NR, Hoare S, Edmonds K, Li NF, Chao D, Hall K, Lee C, Timotheadou E, Charles K, Ahern R, King DM, Eisen T, Corringham R, DeWitte M, Balkwill F, Gore M
    J. Clin. Oncol., 2007;25(29):4542-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: Ctrl
  20. Increase in transforming growth factor-beta in the brain during infection is related to fever, not depression of spontaneous motor activity.
    Authors: Matsumura S, Shibakusa T, Fujikawa T, Yamada H, Inoue K, Fushiki T
    Neuroscience, 2007;144(3):1133-40.
    Species: Rat
    Sample Type: CSF
    Application: Ctrl
  21. Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates.
    Authors: Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ
    J. Immunol., 2004;172(9):5753-64.
    Species: Primate - Macaca mulatta (Rhesus monkey)
    Sample Type: Whole Cells
    Application: Ctrl
  22. Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10.
    Authors: Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA
    J. Immunol., 2004;172(9):5213-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  23. Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2.
    Authors: Viti J, Gulacsi A, Lillien L
    J. Neurosci., 2003;23(13):5919-27.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Ctrl
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Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG PE-conjugated Control

Ctrl IC105P
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Normal Rabbit IgG APC-conjugated Control

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Normal Rabbit IgG Alexa Fluor® 488-conjugated Control

Ctrl IC105G
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Rabbit IgG Alexa Fluor® 750-conjugated Antibody

Ctrl IC1051S
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Rabbit IgG Fluorescein-conjugated Antibody

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Rabbit IgG Alexa Fluor® 594-conjugated Antibody

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Rabbit IgG PE-conjugated Antibody

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Rabbit IgG APC-conjugated Antibody

Ctrl IC1051A
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Rabbit IgG Fluorescein‑conjugated Control Antibody

Ctrl IC105F
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Rabbit IgG Alexa Fluor® 488-conjugated Antibody

Ctrl IC1051G
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Normal Rabbit IgG Alexa Fluor® 700-conjugated Control

Ctrl IC105N
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Rabbit IgG Alexa Fluor® 700-conjugated Antibody

Ctrl IC1051N
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Rabbit IgG PerCP-conjugated Antibody

Ctrl IC1051C
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Rabbit IgG Alexa Fluor® 405-conjugated Antibody

Ctrl IC1051V
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Rabbit IgG Alexa Fluor® 647-conjugated Antibody

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Average Rating: 4.7 (Based on 3 reviews)

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Read what people are saying who have used Normal Rabbit IgG Control.

We have 2 reviews tested in 1 species: Mouse.
We have 3 reviews tested in 2 applications: Functional Assay, Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Functional Assay Mouse Anonymous 07/27/2016
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Summary

ApplicationFunctional Assay
Sample Testedmouse lung
SpeciesMouse
  Excellent
 Flow Mouse Anonymous 06/28/2016
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Summary

ApplicationFlow Cytometry
Sample Testedprimary mouse cells
SpeciesMouse
  Very Good
 Flow Anonymous 02/22/2016
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Summary

ApplicationFlow Cytometry
Sample TestedMouse lymphocytes

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