PON3 Antibody (454929)

R&D Systems | Catalog # MAB4345

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 454929
View all PON3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PON3
Ala30-Leu354
Accession # AAH70374

Specificity

Detects endogenous human, mouse, and rat PON3 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PON1 or rhPON2.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for PON3 Antibody (454929)

Detection of Human/Mouse/Rat PON3 antibody by Western Blot.

Detection of Human/Mouse/Rat PON3 by Western Blot.

Western blot shows lysates of human liver tissue, an enriched HDL /LDL sample obtained from ultracentrifugation of human plasma, mouse liver, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat PON3 Monoclonal Antibody (Catalog # MAB4345) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PON3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for PON3 Antibody (454929)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Human liver tissue, an enriched HDL /LDL sample obtained from ultracentrifugation of human plasma, mouse liver, and rat liver tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PON3

The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/lactonase 3 (PON3) is a 354 amino acid, 39.6 kDa protein that is secreted into the bloodstream and associates with high-density lipoprotein (HDL). PON3 has lactonase activity (EC 3.1.1) and rapidly hydrolyzes lactones. PON3 exhibits no paraoxonase and very limited arylesterase activities. PON3 is an important antioxidant and can inhibit the oxidation of low-density lipoprotein (LDL), a function that may slow the initiation and progression of atherosclerosis. PON3 is glycosylated at Asn323 and alternatively spliced variants encoding different protein isoforms have been described; however, only one has been fully characterized. Human PON3 shares 81% amino acid identity with mouse and rat PON3.

Long Name

Paraoxonase 3

Alternate Names

EC 3.1.1.2, EC 3.1.1.81, EC 3.1.8.1, paraoxanase-3, paraoxonase 3, serum paraoxonase/lactonase 3

Entrez Gene IDs

5446 (Human); 269823 (Mouse); 312086 (Rat)

Gene Symbol

PON3

UniProt

AAH70374

Additional PON3 Products

Product Documents for PON3 Antibody (454929)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PON3 Antibody (454929)

For research use only

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