Detects endogenous human, mouse, and rat PP2A in Western blots. Because PP2A-alpha and PP2A-beta catalytic subunits are identical in the region used for the antigen, it is expected that both isoforms will be detected by the antibody.
Polyclonal Rabbit IgG
E. coli-derived recombinant human PP2A-alpha catalytic subunit Trp209-Leu309 Accession # P67775
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat PP2A Catalytic Subunit, by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line, C2C12 mouse myoblast cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse/Rat PP2A Catalytic Subunit, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1653) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PP2A Catalytic Subunit, at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Protein Phosphatase 2A (PP2A) is an enzyme that removes phosphates covalently attached to serine and threonine residues in proteins. It contains three subunits; a catalytic subunit C (PP2Ac), an assembly/structural subunit A (PP2Aa), and a regulatory subunit B (PP2Ab). PP2A is a ubiquitous enzyme that plays a role in regulating many cellular activities varying from ion transport in erythrocytes to suppressing tumor growth. Binding of the SV40 small T antigen to the PP2A phosphatase complex is known to cause oncogenic transformation.
Entrez Gene IDs:
5515 (Human); 51792 (Mouse)
EC 188.8.131.52; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic subunit, alpha isoform; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.