Human/Mouse/Rat PP2A Catalytic Subunit Antibody

R&D Systems | Catalog # AF1653

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PP2A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PP2A-alpha catalytic subunit
Trp209-Leu309
Accession # P67775

Specificity

Detects endogenous human, mouse, and rat PP2A in Western blots. Because PP2A-alpha and PP2A-beta catalytic subunits are identical in the region used for the antigen, it is expected that both isoforms will be detected by the antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PP2A Catalytic Subunit Antibody

Detection of Human/Mouse/Rat PP2A Catalytic Subunit, antibody by Western Blot.

Detection of Human/Mouse/Rat PP2A Catalytic Subunit, by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse/Rat PP2A Catalytic Subunit, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1653) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PP2A Catalytic Subunit, at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of PP2A Catalytic Subunit by Western Blot

Detection of PP2A Catalytic Subunit by Western Blot

S100A8/A9 proteins augment differentiation and osteoclastic activity derived from iOCPs but not from hOCPs. a Expression of S100A8, S100A9 and RAGE in sorted control and inflamed BM iOCPs and hOCPs. The protein phosphatase 2A catalytic subunit (PP2Ac) is shown as a loading control. b Densitometry measurements of three biological repeats (no statistical analysis presented). c Sorted control and inflamed BM iOCPs and hOCPs (5 × 104) were cultured on the Osteo assay surface with or without anti-RAGE blocking antibodies (bar: 50 µm). d Pit area quantitation. e Sorted iOCPs and hOCPs (5 × 104) from the BM of control mice were cultured on the Osteo assay surface in combination with a recombinant S100A8/A9 heterodimer and anti-RAGE blocking antibodies as indicated (bar: 50 µm). f Pit area quantitation. c–f Depict representative results for two independent experiments, n = 5 for each group. Line: median, box: 25th-75th percentile, whiskers: range. *P < 0.05 (Mann–Whitney test and Holm multiplicity correction) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35396510), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat PP2A Catalytic Subunit Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: U937 human histiocytic lymphoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line

Reviewed Applications

Read 1 review rated 4 using AF1653 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PP2A

Protein Phosphatase 2A (PP2A) is an enzyme that removes phosphates covalently attached to serine and threonine residues in proteins. It contains three subunits; a catalytic subunit C (PP2Ac), an assembly/structural subunit A (PP2Aa), and a regulatory subunit B (PP2Ab). PP2A is a ubiquitous enzyme that plays a role in regulating many cellular activities varying from ion transport in erythrocytes to suppressing tumor growth. Binding of the SV40 small T antigen to the PP2A phosphatase complex is known to cause oncogenic transformation.

Alternate Names

PP2CA, PPP2CA, RP-C

Entrez Gene IDs

5515 (Human); 51792 (Mouse)

Gene Symbol

PPP2CA

UniProt

P67775

Additional PP2A Products

Product Documents for Human/Mouse/Rat PP2A Catalytic Subunit Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PP2A Catalytic Subunit Antibody

For research use only

Citations for Human/Mouse/Rat PP2A Catalytic Subunit Antibody

Customer Reviews for Human/Mouse/Rat PP2A Catalytic Subunit Antibody (1)

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  • Human/Mouse/Rat PP2A Catalytic Subunit Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Prostate cancer
    Species: Human
    Verified Customer | Posted 01/25/2018
    Human/Mouse/Rat PP2A Catalytic Subunit Antibody AF1653

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