Detection of Human, Mouse, and Rat Properdin by Western Blot.
Western blot shows lysates of human serum, mouse serum, and rat serum. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Properdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8216) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Properdin at approximately 53-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human Properdin, also known as Complement factor P (CFP), is a 53 kDa secreted glycoprotein that exists in
plasma as dimers, trimers or tetramers. It a positive regulator of the
alternate pathway of complement.
Properdin binds to and stabilizes
the C3- and C5-convertase enzyme complexes and is thought to connect humoral
and cellular immunity. Properdin released from activated neutrophils may play a
major role in pattern recognition, whereas plasma properdin may primarily
stabilize convertase. Properdin deficiency results in higher susceptibility to
bacterial infections. Full-length human Properdin shares 77 % sequence identity
with mouse Properdin.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.