Human/Mouse/Rat Properdin Antibody

Catalog # Availability Size / Price Qty
AF8216
AF8216-SP
Human/Mouse/Rat Properdin Antibody in Data
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Product Details
Citations (1)
FAQs
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Reviews (1)

Human/Mouse/Rat Properdin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Properdin in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Human embryonic kidney cell line HEK293-derived human Properdin
Asp28-Leu469
Accession # P27918
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human, Mouse, and Rat Properdin by Western Blot. View Larger

Detection of Human, Mouse, and Rat Properdin by Western Blot. Western blot shows lysates of human serum, mouse serum, and rat serum. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Properdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8216) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Properdin at approximately 53-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Properdin

Human Properdin, also known as Complement factor P (CFP), is a 53 kDa secreted  glycoprotein that exists in plasma as dimers, trimers or tetramers. It a positive regulator of the alternate pathway of complement.  Properdin  binds to and stabilizes the C3- and C5-convertase enzyme complexes and is thought to connect humoral and cellular immunity. Properdin released from activated neutrophils may play a major role in pattern recognition, whereas plasma properdin may primarily stabilize convertase. Properdin deficiency results in higher susceptibility to bacterial infections. Full-length human Properdin shares 77 % sequence identity with mouse Properdin.

Entrez Gene IDs
5199 (Human); 18636 (Mouse); 299314 (Rat)
Alternate Names
BFD; CFP; Complement Factor P; complement factor properdin; PFC; PFCcomplement; PFD; Properdin

Product Datasheets

Citation for Human/Mouse/Rat Properdin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Complement inhibition by Sarcoptes scabiei protects Streptococcus pyogenes - An in vitro study to unravel the molecular mechanisms behind the poorly understood predilection of S. pyogenes to infect mite-induced skin lesions
    Authors: PM Swe, LD Christian, HC Lu, KS Sriprakash, K Fischer
    PLoS Negl Trop Dis, 2017;11(3):e0005437.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

FAQs

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Reviews for Human/Mouse/Rat Properdin Antibody

Average Rating: 4 (Based on 1 Review)

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Human/Mouse/Rat Properdin Antibody
By Anonymous on 08/30/2016
Application: WB Sample Tested: Serum Species: Human

Need to load 4 ul of serum to get this quality of WB its not working with 1 UL of serum