Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 399527
View all RalA Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RalA
Met1-Leu206
Accession # P11233

Specificity

Detects human, mouse, and rat RalA in Western blots. In Western blots, no cross-reactivity with recombinant human RalB is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat RalA Antibody

Detection of Human/Mouse/Rat RalA antibody by Western Blot.

Detection of Human/Mouse/Rat RalA by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat RalA Monoclonal Antibody (Catalog # MAB3270) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human RalA and RalB (2 ng/lane) were included. A specific band for RalA was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human/Mouse/Rat RalA Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line and MDA-MB-468 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RalA

The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.

Long Name

Ras-like Protein A

Alternate Names

Rasl1

Entrez Gene IDs

5898 (Human); 56044 (Mouse); 81757 (Rat)

Gene Symbol

RALA

UniProt

P11233

Additional RalA Products

Product Documents for Human/Mouse/Rat RalA Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat RalA Antibody

For research use only

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