Human/Mouse/Rat RUNX1/CBFA2 Antibody

Name: Human/Mouse/Rat RUNX1/CBFA2 Antibody
Brand: R&D Systems
SKU: MAB2399

Catalog #: MAB2399
1 Citation Datasheet / COA / SDS

Discontinued Product

MAB2399 has been discontinued.

View all RUNX1/CBFA2 products.

Detection of Human and Mouse RUNX1/CBFA2 by Western Blot.

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All RUNX1/CBFA2 Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat RUNX1/CBFA2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat CBFA2 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 255019

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human RUNX1/CBFA2
Gly217-Tyr480
Accession # Q01196-8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse RUNX1/CBFA2 by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat RUNX1/CBFA2 Monoclonal Antibody (Catalog # MAB2399) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RUNX1/CBFA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RUNX1/CBFA2

CBFA2, also known as Runt-related Transcription factor 1 (RUNX1), is a heterodimeric transcription factor that plays a role in normal hematopoiesis. Defects in CBFA2 are associated with several types of leukemia.

Long Name

Runt-related Transcription Factor 1

Entrez Gene IDs

861 (Human); 12394 (Mouse); 50662 (Rat)

Alternate Names

Acute myeloid leukemia 1 protein; AML1; AML1acute myeloid leukemia 1; AML1-EVI-1; CBFA2; CBFA2acute myeloid leukemia 1 protein (oncogene AML-1), core-binding factor, alphasubunit10AMLCR1; Core-binding factor subunit alpha-2; EVI-1; PEBP2A2; runt domain, alpha subunit 2; runt-related transcription factor 1; RUNX1; SL3/AKV core-binding factor alpha B subunit

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse/Rat RUNX1/CBFA2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Methylation-independent silencing of the tumor suppressor INK4b (p15) by CBFbeta-SMMHC in acute myelogenous leukemia with inv(16).
Authors: Markus J, Garin MT, Bies J, Galili N, Raza A, Thirman MJ, Le Beau MM, Rowley JD, Liu PP, Wolff L
Cancer Res., 2007-02-01;67(3):992-1000.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot