Detection of Human and Mouse RUNX1/CBFA2 by Western Blot. Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat RUNX1/CBFA2 Monoclonal Antibody (Catalog # MAB2399) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RUNX1/CBFA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CBFA2, also known as Runt-related Transcription factor 1 (RUNX1), is
a heterodimeric transcription factor that plays a role in normal
hematopoiesis. Defects in CBFA2 are associated with several types of
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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