Human/Mouse/Rat RUNX1/CBFA2 Antibody

MAB2399 has been discontinued. View all RUNX1/CBFA2 products.
(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects endogenous human, mouse, and rat CBFA2 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 255019
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human RUNX1/CBFA2
    Gly217-Tyr480
    Accession # Q01196-8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse RUNX1/CBFA2 by Western Blot. Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat RUNX1/CBFA2 Monoclonal Antibody (Catalog # MAB2399) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RUNX1/CBFA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RUNX1/CBFA2
CBFA2, also known as Runt-related Transcription factor 1 (RUNX1), is a heterodimeric transcription factor that plays a role in normal hematopoiesis. Defects in CBFA2 are associated with several types of leukemia.
  • Long Name:
    Runt-related Transcription Factor 1
  • Entrez Gene IDs:
    861 (Human); 12394 (Mouse); 50662 (Rat)
  • Alternate Names:
    Acute myeloid leukemia 1 protein; AML1; AML1acute myeloid leukemia 1; AML1-EVI-1; CBFA2; CBFA2acute myeloid leukemia 1 protein (oncogene AML-1), core-binding factor, alphasubunit10AMLCR1; Core-binding factor subunit alpha-2; EVI-1; PEBP2A2; runt domain, alpha subunit 2; runt-related transcription factor 1; RUNX1; SL3/AKV core-binding factor alpha B subunit
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Methylation-independent silencing of the tumor suppressor INK4b (p15) by CBFbeta-SMMHC in acute myelogenous leukemia with inv(16).
    Authors: Markus J, Garin MT, Bies J, Galili N, Raza A, Thirman MJ, Le Beau MM, Rowley JD, Liu PP, Wolff L
    Cancer Res., 2007;67(3):992-1000.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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