Detection of Human, Mouse, and Rat SEC13 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/ Mouse/Rat SEC13 Monoclonal Antibody (Catalog # MAB90552) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SEC13 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Sec13 is a ubiquitously expressed protein which participates
in the formation of vesicles in the COPII complex with Sec23p.Sec24p, Sar1p and Sec31. The COPII
vesicle budding complex was first described in the yeast system and was shown
to provide coat proteins in the early secretory pathway. Sec13 is recruited to
endoplasmic reticulum (ER) membranes where it forms a three dimensional
cuboctahedron cage lattice structure by association with Sec31. This vesicular
structure is necessary for ER-Golgi transport. Presenillin-1, which is
implicated in early-onset Alzheimer's disease has been shown to interact with
Sec13 vesicles. Additionally, Sec13 has been shown to be a component of
the Y-shaped Nup107-160 subcomplex of the nuclear pore complex (NPC), and the
GATOR2 sub-complex of the GATOR complex.
Nuclear Pore Complex Protein Nup85
Entrez Gene IDs:
6396 (Human); 110379 (Mouse); 297522 (Rat)
D3S1231E; D3S1231ESEC13L1npp-20; Npp-20; protein SEC13 homolog; SEC13 (S. cerevisiae)-like 1; SEC13 homolog (S. cerevisiae); SEC13; SEC13L1; SEC13-like 1 isoform; SEC13-like protein 1; SEC13R; SEC13-related protein; SEC13RSEC13-like 1 (S. cerevisiae)