Human/Mouse/Rat STI1 Antibody

Catalog # Availability Size / Price Qty
AF7337
AF7337-SP
Human/Mouse/Rat STI1 Antibody in Western Blot
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Product Details
Citations (1)
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Human/Mouse/Rat STI1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, rat STI1 in Western blots and detects recombinant human STI1 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human STI1
Met1-Arg543
Accession # P31948
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human, Mouse, and Rat STI1 by Western Blot. View Larger

Detection of Human, Mouse, and Rat STI1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse/Rat STI1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7337) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for STI1 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STI1

STI1 (Stress-induced phosphoprotein 1; also HOP and STIP-1) is a 60-62 kDa member of the STI-1 family of proteins. It is widely expressed, and participates in the formation of functional steroid hormone receptor complexes. Steroid receptors (SR) require proper folding to achieve optimal conformation. In the case of progesterone and glucocorticoid receptors, interaction with chaperones hsp70 and hsp90 facilitates this folding. STI1 is not a chaperone, but a mediator of SR-hsp70-hsp90 interaction. STI1 first binds dimeric hsp90. This trimer then interacts with a SR-(monomeric)hsp70-ADP protein heterodimer to form a SR-hsp70-STI1-hsp90 pentamer that subsequently dissociates into a mature hsp90-SR complex. The STI1-hsp90 complex is also found extracellularly and may contribute to MMP-2 activation. Human STI1 is 543 amino acids (aa) in length. It contains an initial N-terminal TPR domain (aa 4-105) that binds to hsp70, followed by a second TPR domain (aa 225-461) that binds to dimeric hsp90. There are at least 10 acetylated Lys residues plus four utilized phosphorylation sites. Zone potential isoform variant is reported that contains a 48 aa substitution for Gln3. Full-length human STI1 shares 97% aa sequence identity with mouse STI1.

Long Name
Stress-induced Phosphoprotein 1
Entrez Gene IDs
10963 (Human); 20867 (Mouse); 192277 (Rat)
Alternate Names
HOP; HOPSTI1L; Hsc70/Hsp90-organizing protein; Hsp70/Hsp90-organizing protein; IEF-SSP-3521; NY-REN-11 antigen; NY-REN-11; Renal carcinoma antigen NY-REN-11; STI1; STI1L; STI1P60; STIP1; stress-induced-phosphoprotein 1; Transformation-sensitive protein IEF SSP 3521

Product Datasheets

Citation for Human/Mouse/Rat STI1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Exploitation of the Leishmania exosomal pathway by Leishmania RNA virus 1
    Authors: VD Atayde, A da Silva L, V Chaparro, A Zimmermann, C Martel, M Jaramillo, M Olivier
    Nat Microbiol, 2019;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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