Human/Mouse/Rat T Cell Receptor alpha  Chain-V  alpha 24-J  alpha Q Antibody

Catalog # Availability Size / Price Qty
AF4988
AF4988-SP
Detection of Human, Mouse, and Rat T Cell Receptor  alpha  Chain-V  alpha 24-J  alpha Q by Western Blot.
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Human/Mouse/Rat T Cell Receptor alpha  Chain-V  alpha 24-J  alpha Q Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat T Cell Receptor alpha Chain-V alpha 24-J alpha Q in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human T Cell Receptor alpha Chain-V alpha 24-J alpha Q
Lys22-Pro135
Accession # ABC72391
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat T Cell Receptor a Chain-V a24-J aQ antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat T Cell Receptor alpha Chain-V alpha 24-J alpha Q by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and EL-4 mouse lymphoblast cell line, mouse spleen, rat and, mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat T Cell Receptor a Chain-V a24-J aQ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4988) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for T Cell Receptor a Chain-V a24-J aQ at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: T Cell Receptor alpha Chain-V alpha 24-J alpha Q

V alpha 24-J alpha Q (T cell receptor alpha chain) is a 31 kDa (predicted) member of the Ig superfamily. It constitutes an invariant alpha -chain that is paired with the V beta 11 chain on human NKTINV cells, a subpopulation of NKR-P1A T cells. This TCR creates a binding site for CD1d-associated ligands that are presumed to involve glyco‑/phospholipids. The human V alpha 24V beta 11 TCR is analogous to the V alpha 14V beta 8 complex in mice. Human V alpha 24-J alpha Q is 276 amino acids in length. It contains a 21 aa signal sequence, multiple N-linked glycosylation sites, a V-type Ig-like domain (aa 36‑133) and a TCR-related sequence of unknown function (aa 142‑255). Considerable variability is noted over aa 22‑114. The closest mouse ortholog to human V alpha 24-J alpha Q shows 65% aa identity over aa 22‑135.

Alternate Names
T Cell Receptor alpha Chain-V alpha 24-J alpha Q; T CellReceptor alpha ChainV alpha 24J alpha Q

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