Detection of Human, Mouse, and Rat T Cell Receptor alpha Chain-V alpha 24-J alpha Q by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and EL‑4 mouse lymphoblast cell line, mouse spleen, rat and, mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat T Cell Receptor alpha Chain-V alpha 24-J alpha Q Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4988) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for T Cell Receptor alpha Chain-V alpha 24-J alpha Q at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: T Cell Receptor alpha Chain-V alpha 24-J alpha Q
V alpha 24-J alpha Q (T cell receptor alpha chain) is a 31 kDa (predicted) member of the Ig superfamily. It constitutes an invariant alpha -chain that is paired with the V beta 11 chain on human NKTINV cells, a subpopulation of NKR-P1A T cells. This TCR creates a binding site for CD1d-associated ligands that are presumed to involve glyco‑/phospholipids. The human V alpha 24V beta 11 TCR is analogous to the V alpha 14V beta 8 complex in mice. Human V alpha 24-J alpha Q is 276 amino acids in length. It contains a 21 aa signal sequence, multiple N-linked glycosylation sites, a V-type Ig-like domain (aa 36‑133) and a TCR-related sequence of unknown function (aa 142‑255). Considerable variability is noted over aa 22‑114. The closest mouse ortholog to human V alpha 24-J alpha Q shows 65% aa identity over aa 22‑135.
T Cell Receptor alpha Chain-V alpha 24-J alpha Q; T CellReceptor alpha ChainV alpha 24J alpha Q
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