Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all T Cell Receptor alpha Chain-V alpha 24-J alpha Q Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human T Cell Receptor alpha Chain-V alpha 24-J alpha Q
Lys22-Pro135
Accession # ABC72391

Specificity

Detects human, mouse, and rat T Cell Receptor alpha Chain-V alpha 24-J alpha Q in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images

Detection of Human, Mouse, and Rat T Cell Receptor a Chain-V a24-J aQ antibody by Western Blot.

Detection of Human, Mouse, and Rat T Cell Receptor alpha Chain-V alpha 24-J alpha Q by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and EL-4 mouse lymphoblast cell line, mouse spleen, rat and, mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat T Cell Receptor a Chain-V a24-J aQ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4988) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for T Cell Receptor a Chain-V a24-J aQ at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and EL‑4 mouse lymphoblast cell line, mouse spleen, rat and, mouse thymus tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: T Cell Receptor alpha Chain-V alpha 24-J alpha Q

V alpha 24-J alpha Q (T cell receptor alpha chain) is a 31 kDa (predicted) member of the Ig superfamily. It constitutes an invariant alpha -chain that is paired with the V beta 11 chain on human NKTINV cells, a subpopulation of NKR-P1A T cells. This TCR creates a binding site for CD1d-associated ligands that are presumed to involve glyco‑/phospholipids. The human V alpha 24V beta 11 TCR is analogous to the V alpha 14V beta 8 complex in mice. Human V alpha 24-J alpha Q is 276 amino acids in length. It contains a 21 aa signal sequence, multiple N-linked glycosylation sites, a V-type Ig-like domain (aa 36‑133) and a TCR-related sequence of unknown function (aa 142‑255). Considerable variability is noted over aa 22‑114. The closest mouse ortholog to human V alpha 24-J alpha Q shows 65% aa identity over aa 22‑135.

Alternate Names

T CellReceptor alpha ChainV alpha 24J alpha Q

UniProt

ABC72391

Additional T Cell Receptor alpha Chain-V alpha 24-J alpha Q Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices

For research use only

Related Research Areas

T Cell Antigen Recognition

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