Human/Mouse/Rat UBR5 Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat UBR5 by Western Blot.
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Product Details
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Human/Mouse/Rat UBR5 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat UBR5 in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human UBR5
Accession # O95071
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat UBR5 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat UBR5 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HT-2 mouse T cell line, and rat ovary tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat UBR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6404) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UBR5 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UBR5

UBR5 (Ubiquitin protein ligase E3 component n-recognin 5; also HYD and EDD) is a 280‑300 kDa member of the HECT E3 family of ubiquitin (Ub) protein ligases. It is ubiquitously expressed, and found in both the cytoplasm and nucleus of cells. In addition to ubiquination, UBR5 is functionally diverse. In the cytoplasm, it interacts with, and stabilizes APC, thereby increasing its concentration. In the nucleus (of smooth muscle cells), it stabilizes myocardin, a transcription factor that activates smooth muscle-specific genes. Perhaps its best known function involves DNA checkpoint activation. Following DNA damage, UBR5 promotes the phosphorylation of CHK2, which arrests the cell cycle at G2/M. Human UBR5 is 2799 amino acids (aa) in length and contains a Ub-associated domain (aa 180‑215), two NLS's (aa 502‑517 and 630‑635), one UBR-type zinc finger region (aa 1177‑1244), a PABC region (aa 2377‑2454) and a HECT domain with a Ub-binding Cys residue (aa 2462‑2799). Over aa 1‑227, human UBR5 shares 98% aa identity with mouse UBR5.

Long Name
Ubiquitin Protein Ligase E3 Component N-recognin 5
Entrez Gene IDs
51366 (Human); 70790 (Mouse); 117060 (Rat)
Alternate Names
DD5; E3 ubiquitin protein ligase, HECT domain containing, 1; E3 ubiquitin-protein ligase, HECT domain-containing 1; EC 6.3.2; EC 6.3.2.-; EDD; EDD1; EDD1E3 ubiquitin-protein ligase UBR5; EDDE3 identified by differential display; hHYD; HYD; HYDFLJ11310; Hyperplastic discs protein homolog; KIAA0896MGC57263; progestin induced protein; Progestin-induced protein; ubiquitin protein ligase E3 component n-recognin 5; ubiquitin-protein ligase; UBR5

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