Human/Mouse/Rat UBR5 Antibody Summary
Accession # O95071
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat UBR5 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HT-2 mouse T cell line, and rat ovary tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat UBR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6404) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UBR5 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
UBR5 (Ubiquitin protein ligase E3 component n-recognin 5; also HYD and EDD) is a 280‑300 kDa member of the HECT E3 family of ubiquitin (Ub) protein ligases. It is ubiquitously expressed, and found in both the cytoplasm and nucleus of cells. In addition to ubiquination, UBR5 is functionally diverse. In the cytoplasm, it interacts with, and stabilizes APC, thereby increasing its concentration. In the nucleus (of smooth muscle cells), it stabilizes myocardin, a transcription factor that activates smooth muscle-specific genes. Perhaps its best known function involves DNA checkpoint activation. Following DNA damage, UBR5 promotes the phosphorylation of CHK2, which arrests the cell cycle at G2/M. Human UBR5 is 2799 amino acids (aa) in length and contains a Ub-associated domain (aa 180‑215), two NLS's (aa 502‑517 and 630‑635), one UBR-type zinc finger region (aa 1177‑1244), a PABC region (aa 2377‑2454) and a HECT domain with a Ub-binding Cys residue (aa 2462‑2799). Over aa 1‑227, human UBR5 shares 98% aa identity with mouse UBR5.
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