Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all UBR5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human UBR5
Met1-Asp227
Accession # O95071

Specificity

Detects human, mouse, and rat UBR5 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat UBR5 Antibody

Detection of Human, Mouse, and Rat UBR5 antibody by Western Blot.

Detection of Human, Mouse, and Rat UBR5 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HT-2 mouse T cell line, and rat ovary tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat UBR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6404) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UBR5 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse/Rat UBR5 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, HT‑2 mouse T cell line, and rat ovary tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBR5

UBR5 (Ubiquitin protein ligase E3 component n-recognin 5; also HYD and EDD) is a 280‑300 kDa member of the HECT E3 family of ubiquitin (Ub) protein ligases. It is ubiquitously expressed, and found in both the cytoplasm and nucleus of cells. In addition to ubiquination, UBR5 is functionally diverse. In the cytoplasm, it interacts with, and stabilizes APC, thereby increasing its concentration. In the nucleus (of smooth muscle cells), it stabilizes myocardin, a transcription factor that activates smooth muscle-specific genes. Perhaps its best known function involves DNA checkpoint activation. Following DNA damage, UBR5 promotes the phosphorylation of CHK2, which arrests the cell cycle at G2/M. Human UBR5 is 2799 amino acids (aa) in length and contains a Ub-associated domain (aa 180‑215), two NLS's (aa 502‑517 and 630‑635), one UBR-type zinc finger region (aa 1177‑1244), a PABC region (aa 2377‑2454) and a HECT domain with a Ub-binding Cys residue (aa 2462‑2799). Over aa 1‑227, human UBR5 shares 98% aa identity with mouse UBR5.

Long Name

Ubiquitin Protein Ligase E3 Component N-recognin 5

Alternate Names

DD5, EDD, EDD1, HYD

Entrez Gene IDs

51366 (Human); 70790 (Mouse); 117060 (Rat)

Gene Symbol

UBR5

UniProt

O95071 (Human)

Additional UBR5 Products

Product Documents for Human/Mouse/Rat UBR5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat UBR5 Antibody

For research use only

Related Research Areas

E3 Ubiquitin Ligases

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