Human/Mouse/Rat WWOX Antibody

Catalog # Availability Size / Price Qty
AF6399
AF6399-SP

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Detection of Human, Mouse, and Rat WWOX by Western Blot.
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Product Details
Citations (2)
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Human/Mouse/Rat WWOX Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat WWOX in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human WWOX
Asn36-Val126
Accession # Q9NZC7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat WWOX antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat WWOX by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat WWOX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6399) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for WWOX at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western Blot Detection of Human WWOX by Western Blot View Larger

Detection of Human WWOX by Western Blot Intracellular distribution of Wwox, Dvl-1, Dvl-2 and Dvl-3 proteins in HET-1A and SCC-15 cell lines. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32368285), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WWOX

WWOX (WW domain-containing oxidoreductase; also WOX1 and FOR) is a 46 kDa cytoplasmic member of the short-chain dehydrogenases/reductases (or SDR) family of molecules. It is expressed in divergent cell types such as breast epithelium, keratinocytes, osteoblasts, and osteoclasts. WWOX has multiple effects, one of which is that of tumor suppressor. Here, WWOX is likely activated by either TGF-beta binding to surface hyaluronoglucosaminidase 2, or C1q binding to C1qR, which, in both cases, initiates cell apoptosis. Human WWOX is 414 amino acids (aa) in length and contains two WW (TrpTrp) domains (aa 16‑90) with an intervening NLS (aa 50‑55), plus an SDR region that contains a mitochondrial targeting sequence (aa 121‑330). There are multiple phosphorylation sites and multiple splice variants that impact intracellular localization. One shows a deletion of aa 173‑352, a second shows a Lys substitution for aa 36‑414, a third contains a 17 aa substitution for
aa 173‑414, a fourth possesses a 76 aa substitution for aa 138‑414, a fifth shows an 11 aa substitution for aa 353‑414, while a sixth contains a 175 aa substitution for aa 137‑414. Over aa 36‑126, human and mouse WWOX are identical in aa sequence.

Long Name
WW Domain Containing Oxidoreductase
Entrez Gene IDs
51741 (Human); 80707 (Mouse); 292041 (Rat)
Alternate Names
D16S432E; EC 1.1.1; EC 1.1.1.-; FOR; FORHHCMA56; FRA16D; Fragile site FRA16D oxidoreductase; SDR41C1; short chain dehydrogenase/reductase family 41C, member 1; WOX1; WOX1PRO0128; WW domain containing oxidoreductase; WW domain-containing oxidoreductase; WW domain-containing protein WWOX; WWOX

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Citations for Human/Mouse/Rat WWOX Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. B-cell receptor associated protein 31 deficiency decreases the expression of adhesion molecule CD11b/CD18 and PSGL-1 in neutrophils to ameliorate acute lung injury
    Authors: Li GX, Jiang XH, Zang JN et al.
    The International Journal of Biochemistry & Cell Biology
  2. Silencing of Wwox Increases Nuclear Import of Dvl proteins in Head and Neck Cancer
    Authors: A Celebi, C Orhan, B Seyhan, N Buyru
    J Cancer, 2020-04-06;11(14):4030-4036.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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