Detection of Human, Mouse, and Rat WWOX by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat WWOX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6399) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for WWOX at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
WWOX (WW domain-containing oxidoreductase; also WOX1 and FOR) is a 46 kDa cytoplasmic member of the short-chain dehydrogenases/reductases (or SDR) family of molecules. It is expressed in divergent cell types such as breast epithelium, keratinocytes, osteoblasts, and osteoclasts. WWOX has multiple effects, one of which is that of tumor suppressor. Here, WWOX is likely activated by either TGF-beta binding to surface hyaluronoglucosaminidase 2, or C1q binding to C1qR, which, in both cases, initiates cell apoptosis. Human WWOX is 414 amino acids (aa) in length and contains two WW (TrpTrp) domains (aa 16‑90) with an intervening NLS (aa 50‑55), plus an SDR region that contains a mitochondrial targeting sequence (aa 121‑330). There are multiple phosphorylation sites and multiple splice variants that impact intracellular localization. One shows a deletion of aa 173‑352, a second shows a Lys substitution for aa 36‑414, a third contains a 17 aa substitution for aa 173‑414, a fourth possesses a 76 aa substitution for aa 138‑414, a fifth shows an 11 aa substitution for aa 353‑414, while a sixth contains a 175 aa substitution for aa 137‑414. Over aa 36‑126, human and mouse WWOX are identical in aa sequence.
WW Domain Containing Oxidoreductase
Entrez Gene IDs:
51741 (Human); 80707 (Mouse); 292041 (Rat)
D16S432E; EC 1.1.1; EC 1.1.1.-; FOR; FORHHCMA56; FRA16D; Fragile site FRA16D oxidoreductase; SDR41C1; short chain dehydrogenase/reductase family 41C, member 1; WOX1; WOX1PRO0128; WW domain containing oxidoreductase; WW domain-containing oxidoreductase; WW domain-containing protein WWOX; WWOX
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