Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC3765A

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Detection of RUNX3/CBFA3 in Human PBMC lymphocytes by Flow Cytometry.
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Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse RUNX3/CBFA3 in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 527327
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human RUNX3/CBFA3
Lys186-Tyr415
Accession # Q13761
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of RUNX3/CBFA3 antibody in Human PBMC lymphocytes antibody by Flow Cytometry. View Larger

Detection of RUNX3/CBFA3 in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cells (PBMC) lymphocytes either (A) untreated or (B) treated with PMA were stained with Mouse Anti-Human/Mouse RUNX3/CBFA3 APC-conjugated Monoclonal Antibody (Catalog # IC3765A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: RUNX3/CBFA3

RUNX3, also known as CBFA3, AML-2 or PEBP2-alpha C, is a member of the Runt domain family of nuclear transcriptional regulators. All of the RUNX proteins form dimers with CBF-beta. The Runt domain, amino acids (aa) 54-186, is required for DNA binding, while a pro/ser/thr-rich region (aa 191-415) transcriptionally activates target genes. Isoform 2 has an alternate 19 aa in place of the N-terminal 5 aa of isoform 1. The 415 aa Human RUNX3 shares 91% aa identity with mouse or rat RUNX3. RUNX3 is necessary for growth control of gastric epithelium, neurogenesis of dorsal root ganglia, and T cell differentiation. RUNX3 expression is frequently mutated in tumors and appears to be silenced by methylation.

Long Name
Runt-related Transcription Factor 3
Entrez Gene IDs
864 (Human); 12399 (Mouse); 156726 (Rat)
Alternate Names
Acute myeloid leukemia 2 protein; AML2; AML2SL3/AKV core-binding factor alpha C subunit; CBFA3; CBFA3MGC16070; CBF-alpha-3; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2A3; PEBP2A3FLJ34510; PEBP2AC; PEBP2-alpha C; runt domain, alpha subunit 3; runt-related transcription factor 3; RUNX3; SL3-3 enhancer factor 1 alpha C subunit; transcription factor AML2

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Citation for Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Langerhans cells are generated by two distinct PU.1-dependent transcriptional networks.
    Authors: Chopin M, Seillet C, Chevrier S, Wu L, Wang H, Morse H, Belz G, Nutt S
    J Exp Med, 2013;210(13):2967-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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