Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all SAM68 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human and mouse SAM68
Met108-Pro280
Accession # Q07666

Specificity

Detects human and mouse SAM68 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse SAM68 Antibody

Detection of Human and Mouse SAM68 antibody by Western Blot.

Detection of Human and Mouse SAM68 by Western Blot.

Western blot shows nuclear extracts of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse SAM68 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5509) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SAM68 at approximately 60-68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse SAM68 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SAM68

SAM68 (Src associated in mitosis 68 kDa; also KH domain-containing, RNA-binding, signal transduction-associated protein 1) is a 62‑68 kDa member of the KHDRBS family of proteins. It is associated with multiple receptor systems (i.e.- TCR and leptin), as an adaptor protein by its SH2 and SH3 domains, and binds to UAAA motifs in mRNA. Human SAM68 is 443 amino acids (aa) in length. It contains one SGQ region (aa 96‑157) and one KH domain (aa 171‑197). Post‑translational modification of SAM68 alters its partnership with other molecules. For example, methylation on ArgGly motifs reduce RNA binding, acetylation on multiple Lys residues increases RNA binding, and phosphorylation on Tyr promotes SH3 domain interaction.

Long Name

Src-associated in Mitosis 68 kDa Protein

Alternate Names

KHDRBS1, p62, p68

Entrez Gene IDs

10657 (Human); 20218 (Mouse); 117268 (Rat)

Gene Symbol

KHDRBS1

UniProt

Q07666

Additional SAM68 Products

Product Documents for Human/Mouse SAM68 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse SAM68 Antibody

For research use only

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