Human/Mouse Semaphorin 3A APC-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human Semaphorin 3A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Semaphorin 3B, 3E, 6A, 6B, 6C, 6D, 7A, recombinant mouse Semaphorin 3C, or 3F is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 215803
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Semaphorin 3A
    Lys26-Val771 (Arg555Ala, Arg552Ala)
    Accession # Q14563
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Semaphorin 3A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were stained with Mouse Anti-Human/Mouse Semaphorin 3A APC‑conjugated Monoclonal Antibody (Catalog # IC1250A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of Semaphorin 3A in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Mouse Anti-Human/Mouse Semaphorin 3A APC‑conjugated Monoclonal Antibody (Catalog # IC1250A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Semaphorin 3A

The Semaphorins constitute a large family of secreted, GPI-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 through 7) have been identified. All Semaphorins contain a conserved, 500 amino acid (aa) Sema domain at the amino terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. Semaphorins are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2).

Class 3 Semaphorins (Sema3) are secreted proteins containing a Sema domain, an immunoglobulin c2-like domain and a basic domain near the carboxyl tail. Sema3A (also referred to as SemaIII, SemD and Collapsin) cDNA predicts a 771 aa precursor protein with a putative 25 aa signal peptide (1‑3). Bioactive Sema3A is a disulfide-linked dimer (4). The bioactivity is increased after proteolytic processing by a furin-like endoprotease near the carboxy-terminus (1). The functional receptor complex for Sema3 is composed of two distinct transmembrane proteins: Neuropilin-1 (Npn-1) and Plexin-A. Npn-1 binds directly to Sema3A with high-affinity and confers specificity. Plexin-A interacts with Npn-1 to increase the affinity of the complex for Sema3A and serves as the signaling subunit in the receptor complex (1, 2, 5).

  • References:
    1. Nakamura, F. et al. (2000) J Neurobiol. 44:219.
    2. Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.
    3. Kolodkin, A.L. et al. (1993) Cell 75:1389.
    4. Koppel, A.M. et al. (1998) J. Biol. Chem. 273:15708.
    5. Yu, T.W. et al. (2001) Nature Neurosci. Supplement 4:1169.
    6. Luo Y. et al. (1993) Cell 75:217.
  • Entrez Gene IDs:
    10371 (Human); 102126862 (Cynomolgus Monkey)
  • Alternate Names:
    (semaphorin) 3A; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema III; SEMA1; Sema3A; SEMAD; SEMAIII; SEMAL; Semaphorin 3A; semaphorin D; Semaphorin III; semaphorin L; semaphorin-3A; SemD
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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