Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all SMOC-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SMOC-2 
Gln22-Gly446
Accession # Q9H3U7

Specificity

Detects human and mouse SMOC‑2 in direct ELISAs and Western Blots

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human/Mouse SMOC‑2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human SMOC‑2 (Catalog # 5140-SM)
Recombinant Mouse SMOC‑2 (Catalog # 6075-SM)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SMOC-2

SMOC-2 (secreted modular calcium-binding protein 2; also SMAP2) is a 55 kDa glycoprotein member of the SPARC family of matricellular proteins. It is widely expressed, perhaps by endothelium and apparently participates in both growth factor signaling and ECM organization. Mature human SMOC-2 is 425 amino acids (aa) in length. It contains one Kazal-like domain (aa 40‑84), two thyroglobulin type-1 segments (aa 87‑153 and 213‑281) and two EF-hand sequences (aa 347‑382 and 384‑419). There are three splice variants. One shows a 13 aa substitution for aa 442‑446, another shows an 11 aa insertion after Thr170 and a third represents a combination of the above two variations. Over aa 22‑446, human SMOC-2 shares 95% aa identity with mouse SMOC-2.

Long Name

Secreted Modular Calcium-binding Protein 2

Alternate Names

MST117, MSTP117, MSTP140, SMAP2, SMOC2

Entrez Gene IDs

64094 (Human); 64074 (Mouse); 292401 (Rat)

Gene Symbol

SMOC2

UniProt

Q9H3U7

Additional SMOC-2 Products

Product Documents for Human/Mouse SMOC‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse SMOC‑2 Antibody

For research use only

Citations for Human/Mouse SMOC‑2 Antibody

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